迈向荧光显微镜和光镊中分子运动的控制：一种时空方法。
Towards controlling molecular motions in fluorescence microscopy and optical trapping: a spatiotemporal approach.
作者信息
Kumar De Arijit, Goswami Debabrata
机构信息
Department of Chemistry, Indian Institute of Technology, Kanpur UP-208016, India ; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA ; Department of Chemistry, University of California at Berkeley, Berkeley, CA 94720, USA.
出版信息
Int Rev Phys Chem. 2011 Sep 26;30(3):275-299. doi: 10.1080/0144235X.2011.603237.
This account reviews some recent studies pursued in our group on several control experiments with important applications in (one-photon) confocal and two-photon fluorescence laser-scanning microscopy and optical trapping with laser tweezers. We explore the simultaneous control of internal and external (i.e. centre-of-mass motion) degrees of freedom, which require the coupling of various control parameters to result in the spatiotemporal control. Of particular interest to us is the implementation of such control schemes in living systems. A live cell is a system of a large number of different molecules which combine and interact to generate complex structures and functions. These combinations and interactions of molecules need to be choreographed perfectly in time and space to achieve intended intra-cellular functions. Spatiotemporal control promises to be a versatile tool for dynamical control of spatially manipulated bio-molecules.
本报告回顾了我们小组最近进行的一些对照实验研究，这些研究在（单光子）共聚焦和双光子荧光激光扫描显微镜以及激光镊子光镊等方面有着重要应用。我们探索了对内部和外部（即质心运动）自由度的同时控制，这需要耦合各种控制参数以实现时空控制。我们特别感兴趣的是在生物系统中实施此类控制方案。活细胞是由大量不同分子组成的系统，这些分子相互结合并相互作用，以产生复杂的结构和功能。分子的这些组合和相互作用需要在时间和空间上完美编排，以实现预期的细胞内功能。时空控制有望成为一种用于动态控制空间操纵生物分子的通用工具。
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