Departamento de Ingenieria Electronica, Universitat Rovira i Virgili, Avda. Països Catalans 26, 43007 Tarragona, Spain.
J Am Chem Soc. 2013 Jul 17;135(28):10314-7. doi: 10.1021/ja405120x. Epub 2013 Jul 5.
A surface-enhanced Raman scattering (SERS)-based sensor was developed for the detection of the oncoprotein c-Jun at nanomolar levels. c-Jun is a member of the bZIP (basic zipper) family of dimeric transcriptional activators, and its overexpression has been associated with carcinogenic mechanisms in several human cancers. For our sensing purpose, we exploited the ability of c-Jun to heterodimerize with its native protein partner, c-Fos, and therefore designed a c-Fos peptide receptor chemically modified to incorporate a thiophenol (TP) group at the N-terminal site. The TP functionality anchors the c-Fos protein onto the metal substrate and works as an effective SERS probe to sense the structural rearrangements associated with the c-Fos/c-Jun heterodimerization.
开发了一种基于表面增强拉曼散射（SERS）的传感器，用于检测纳摩尔水平的癌蛋白 c-Jun。c-Jun 是 bZIP（碱性拉链）家族二聚体转录激活因子的成员，其过表达与多种人类癌症的致癌机制有关。为了实现我们的传感目的，我们利用了 c-Jun 与天然蛋白伴侣 c-Fos 形成异二聚体的能力，并因此设计了一种化学修饰的 c-Fos 肽受体，在 N 端位点引入了巯基苯（TP）基团。TP 功能将 c-Fos 蛋白锚定在金属基底上，并作为有效的 SERS 探针，用于检测与 c-Fos/c-Jun 异二聚化相关的结构重排。
