快速检测肠杆菌科中与氟喹诺酮类药物耐药相关的gyrA 和 parC 突变的方法。
Rapid assay for detecting gyrA and parC mutations associated with fluoroquinolone resistance in Enterobacteriaceae.
机构信息
Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan.
出版信息
J Microbiol Methods. 2013 Sep;94(3):213-6. doi: 10.1016/j.mimet.2013.06.019. Epub 2013 Jun 28.
PMID:23816531
Abstract
We developed a PCR-RFLP assay to detect mutations in the quinolone-resistance determining regions of gyrA and parC associated with fluoroquinolone resistance in Enterobacteriaceae. The assay detected mutations associated with reduced susceptibility to fluoroquinolones and may therefore serve as a specific, rapid, inexpensive, and simple testing alternative.
摘要
我们开发了一种 PCR-RFLP 检测方法，用于检测与肠杆菌科中氟喹诺酮类药物耐药相关的 gyrA 和 parC 喹诺酮耐药决定区的突变。该检测方法可检测与氟喹诺酮类药物敏感性降低相关的突变，因此可以作为一种特异性、快速、廉价且简单的替代检测方法。
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