Biomass Energy and Environmental Engineering Research Center, Beijing University of Chemical Technology, 503 Zonghe Building, 15 North 3rd Ring East Road, Beijing, 100029, People's Republic of China.
Appl Biochem Biotechnol. 2013 Sep;171(1):117-27. doi: 10.1007/s12010-013-0335-7. Epub 2013 Jul 2.
In order to investigate the effects of inoculum source and pre-incubation on methane production performance of chicken manure (CM) and corn stover (CS), two sets of bio-methane potential tests using non- and pre-incubated inocula (digested sludge from a municipal wastewater treatment plant (DSMW) and digested sludge from a chicken manure treatment plant (DSCM)) were conducted at 37 °C. Modified Gompertz and first-order models were used to evaluate the kinetic parameters. The results revealed that DSMW was better than DSCM in digesting organic substrates (CM and CS), since the average ultimate methane yields were 351 mL g(-1) volatile solid (VS)added for CM and 300 mL g(-1) VSadded for CS when DSMW was used as inoculum, and 298 mL g(-1) VSadded for CM and 218 mL g(-1) VSadded for CS when DSCM was used as inoculum, respectively. Nevertheless, there was no significant difference (p > 0.05) in the ultimate methane yields between non- and pre-incubated inoculum for digesting CM and CS, regardless of the inoculum source. However, when evaluating the kinetic parameters of anaerobic digestion, the correlation coefficient, maximal methane production rate, and hydrolysis rate constant were slightly higher using pre-incubated inoculum as compared to non-incubated inoculum.
为了研究接种物来源和预孵育对鸡粪（CM）和玉米秸秆（CS）甲烷生成性能的影响，在 37°C 下使用非预孵育和预孵育接种物（城市污水处理厂消化污泥（DSMW）和鸡粪处理厂消化污泥（DSCM））进行了两组生物甲烷潜能测试。使用修正的 Gompertz 和一级模型评估了动力学参数。结果表明，DSMW 在消化有机底物（CM 和 CS）方面优于 DSCM，因为当使用 DSMW 作为接种物时，CM 的平均最终甲烷产量为 351 mL g(-1) 挥发性固体（VS）添加，CS 为 300 mL g(-1) VS 添加，而当使用 DSCM 作为接种物时，CM 的最终甲烷产量为 298 mL g(-1) VS 添加，CS 为 218 mL g(-1) VS 添加。然而，无论接种物来源如何，非预孵育和预孵育接种物消化 CM 和 CS 的最终甲烷产量之间没有显著差异（p > 0.05）。然而，在评估厌氧消化的动力学参数时，与非孵育接种物相比，预孵育接种物的相关系数、最大甲烷生成速率和水解速率常数略高。
