Jørgensen J C, Sejrsen P
Department of Obstetrics and Gynecology, Rigshospitalet, University of Copenhagen, Denmark.
Peptides. 1990 May-Jun;11(3):451-4. doi: 10.1016/0196-9781(90)90041-3.
Neuropeptide Y-containing nerve fibers have previously been demonstrated to innervate the mammalian ovary. These nerve fibers innervate primarily the vasculature. In this study we have developed a method for in vivo measurement of the ovary blood flow rate by means of the 133Xe method. Using this technique we measured the ovary blood flow rate and investigated the dose-response relationship between close intraarterial-injected NPY and the ovary blood flow rate. A monoexponential washout curve for 133Xe was found for the whole washout process, ensuring that the blood flow rate at any time could be calculated from the curve. We found a mean blood flow rate in the nonpregnant rabbit ovary at 43.6 +/- 4.4 ml.(100 g)-1.min-1 (mean +/- SEM). Injection of NPY (20, 200, 2000 pM) in the aorta close to a. ovarica resulted in a dose-dependent decrease in the ovarian blood flow rate with a maximum reduction to 40.7 +/- 6.3% (mean +/- SEM) of the control blood flow rate. These findings make it likely that receptors able to interact with NPY are present in the vasculature of the rabbit ovary.
先前已证明含神经肽Y的神经纤维支配哺乳动物的卵巢。这些神经纤维主要支配脉管系统。在本研究中，我们开发了一种通过133Xe方法体内测量卵巢血流速率的方法。使用该技术，我们测量了卵巢血流速率，并研究了经动脉内近距离注射神经肽Y与卵巢血流速率之间的剂量反应关系。在整个洗脱过程中发现了133Xe的单指数洗脱曲线，确保可以从该曲线计算出任何时间的血流速率。我们发现未怀孕兔子卵巢的平均血流速率为43.6 +/- 4.4 ml·(100 g)-1·min-1（平均值 +/- 标准误）。在靠近卵巢动脉处的主动脉中注射神经肽Y（20、200、2000 pM）导致卵巢血流速率呈剂量依赖性降低，最大降低至对照血流速率的40.7 +/- 6.3%（平均值 +/- 标准误）。这些发现表明，兔卵巢脉管系统中可能存在能够与神经肽Y相互作用的受体。
