Greer J
Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, Illinois 60064.
Proteins. 1990;7(4):317-34. doi: 10.1002/prot.340070404.
Comparative modeling methods are described that can be used to construct a three-dimensional model structure of a new protein from knowledge of its sequence and of the experimental structures and sequences of other members of its homology family. The methods are illustrated with the mammalian serine protease family, for which seven experimental structures have been reported in the literature, and the sequences for over 35 different protein members of the family are available. The strategy for modeling these proteins is presented, and criteria are developed for determining and assigning the reliability of the modeled structure. Criteria are described that are specially designed to help detect cases in which it is likely that the local structure diverges significantly from the usual conformation of the family.
描述了比较建模方法，这些方法可用于根据新蛋白质的序列以及其同源家族其他成员的实验结构和序列知识构建其三维模型结构。以哺乳动物丝氨酸蛋白酶家族为例说明了这些方法，该家族在文献中已报道了七个实验结构，并且可获得该家族35种以上不同蛋白质成员的序列。介绍了对这些蛋白质进行建模的策略，并制定了用于确定和分配建模结构可靠性的标准。还描述了专门设计用于帮助检测局部结构可能与家族通常构象有显著差异情况的标准。
