Zhang Wei-Dong, Wang Pi-Min, Han Run-Xia, Zhang Tian-Sheng
Shanxi College of TCM, Taiyuan 030024, China.
Zhongguo Zhen Jiu. 2013 Apr;33(4):334-8.
To explore the therapeutic effect and safety of fire needling on rat with rheumatoid arthritis.
Forty female Wistar rats were randomly divided into a normal group, a model group, a methotrexate (MTX) group and a fire needling group, 10 rats in each group. The 0.1 mL of normal saline was subcutaneously injected in rat's right back paw in the normal group, while the other groups received subcutaneous injection of 0.1 mL of complete Freund's adjuvant to establish the model. The pricking method was applied in the fire needling group at "Jiaji" (EX-B 2), "Zusanli" (ST 36) and Ashi points with a depth of 5 mm, once every three days, and totally eight times were required. In the MTX group, with intragastric administration of 2.0 mg/kg per rat, the treatment was given once every seven days, and totally four times were required. The weight, swelling rate of foot, joint pain score and polyarthritis index of rats in each group as well as the pathological change of liver tissue under light microscope were observed.
The weight, swelling rate of foot, joint pain score and polyarthritis index in the model group were significantly higher than those in the normal group (P < 0.05, P < 0.01), after the treatment, the above-mentioned indexes in the two treatment groups were obviouly dereased as componed with those in the model group (all P < 0.01), and there were statistical differences between the fire needling group and MTX group (P < 0.05, P < 0.01). Compared with the normal group, inflammatory cells were appeared with a cluster distribution in the liver cells of rats in the model group. The inflammatory cells were reduced in the MTX group and fire needling group, and liver cells had degenerative edema and cell nucleus were largen and deformed in the MTX group.
The fire needling has significant efficacy for rats with adjuvant arthritis (AA) without any damage to the liver, which coud have a better control of disease progression of rheumatoid arthritis .
探讨火针治疗类风湿关节炎大鼠的疗效及安全性。
将40只雌性Wistar大鼠随机分为正常组、模型组、甲氨蝶呤（MTX）组和火针组，每组10只。正常组大鼠右后足皮下注射0.1 mL生理盐水，其余各组皮下注射0.1 mL完全弗氏佐剂建立模型。火针组采用针刺法针刺“夹脊”（EX - B 2）、“足三里”（ST 36）及阿是穴，进针深度5 mm，每3天1次，共需针刺8次。MTX组大鼠按2.0 mg/kg灌胃给药，每7天给药1次，共需给药4次。观察各组大鼠的体重、足肿胀率、关节疼痛评分、多关节炎指数以及光镜下肝组织的病理变化。
模型组大鼠的体重、足肿胀率、关节疼痛评分和多关节炎指数均显著高于正常组（P < 0.05，P < 0.01），治疗后，两个治疗组的上述指标与模型组相比均明显降低（均P < 0.01），且火针组与MTX组之间存在统计学差异（P < 0.05，P < 0.01）。与正常组相比，模型组大鼠肝细胞内出现炎性细胞呈簇状分布。MTX组和火针组炎性细胞减少，MTX组肝细胞有变性水肿，细胞核增大、变形。
火针治疗佐剂性关节炎（AA）大鼠有显著疗效，且对肝脏无损伤，能较好地控制类风湿关节炎的病情进展。
