Du Xiaozheng, Yuan Bo, Wang Jinhai, Zhang Xinghua, Tian Liang, Zhang Tingzhuo, Li Xinglan, Zhang Fengfan
School of Acupuncture-Moxibustion and Tuina, Gansu University of CM, Lanzhou 730000, China.
Department of TCM, the Second Hospital of Lanzhou University.
Zhongguo Zhen Jiu. 2017 Sep 12;37(9):977-83. doi: 10.13703/j.0255-2930.2017.09.018.
To explore heat-reinforcing needling for the metabolite profiling changes in serum of rheumatoid arthritis (RA) rabbits with liquid chromatograph-mass spectrometer (LC-MS) technique, and to investigate its mechanisms.
Forty clean purple blue rabbits were randomized into a normal group, a model group, a reinforcing-reducing needling (RRN) group, a twirling-reinforcing needling (TRN) group, and a heat-reinforcing needling (HRN) group, 8 cases in each group. RA rabbits with cold syndrome were made with ovalbumin and freezing except those in the normal group. No treatment was given in the normal and model groups. The corresponding manipulations for 7 days were applied at "Zusanli" (ST 36) in the three acupuncture groups, 30 min a time, once a day. After intervention the pain threshold and the local skin temperature of each group were observed. Fresh serum from heart was collected for metabonomics detection. Principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were adopted. Several metabolites were screened by the variable importance in the projection values (VIP>1) and value (<0.05).
The pain threshold and the local skin temperature in the model group were lower than those in the normal group (both <0.05). The pain threshold and the local skin temperature in the three acupuncture groups were higher than those in the model group after intervention (all <0.05), which were better in the HRN group than those in the RRN and TRN groups (all <0.05). The serum metabolites of carnitine, LysoPC (14∶0), LysoPC (18∶3), LysoPE (0∶0/20∶5), LysoPE (0∶0/22∶1), decylic acid, stearic acid and lactic acid in the model group increased compared with those in the normal group, and other metabolites decreased, including leucine, valine, glutamine, pyroglutamic acid, α-ketoglutaric acid, succinic acid, fumaric acid, malic acid, galactose, mannose. Those metabolites were correlated fatty acid, amino acid, citric acid cycle, and glucose metabolism. The metabolites above-mentioned in the three acupuncture groups were regulated in various degrees (all <0.05). Lactic acid decreased and succinic acid, fumaric acid, malic acid, galactose, mannose increased more obviously in the HRN group than those in the RRN and TRN groups.
The specificity of heat-reinforcing needling for RA presents the regulation for citric acid cycle and glucose metabolism.
采用液相色谱 - 质谱联用（LC - MS）技术探讨温针治疗对类风湿关节炎（RA）兔血清代谢物谱变化的影响，并探讨其作用机制。
将40只清洁级青紫蓝兔随机分为正常组、模型组、提插补泻针法组（RRN组）、捻转补法组（TRN组）和温针组（HRN组），每组8只。除正常组外，其余各组采用卵白蛋白和冷冻法制备寒证RA兔模型。正常组和模型组不做处理。三个针刺组在“足三里”（ST 36）穴进行相应手法干预7天，每次30分钟，每日1次。干预后观察各组的痛阈和局部皮肤温度。采集心脏新鲜血清进行代谢组学检测。采用主成分分析（PCA）和偏最小二乘判别分析（PLS - DA）。通过变量投影重要性值（VIP>1）和P值（<0.05）筛选出几种代谢物。
模型组的痛阈和局部皮肤温度低于正常组（均P<0.05）。干预后，三个针刺组的痛阈和局部皮肤温度均高于模型组（均P<0.05），其中温针组优于提插补泻针法组和捻转补法组（均P<0.05）。与正常组相比，模型组血清中肉碱、溶血磷脂酰胆碱（14∶0）、溶血磷脂酰胆碱（18∶3）、溶血磷脂酰乙醇胺（0∶0/20∶5）、溶血磷脂酰乙醇胺（0∶0/22∶1）、癸酸、硬脂酸和乳酸等代谢物含量升高，其他代谢物含量降低，包括亮氨酸、缬氨酸、谷氨酰胺、焦谷氨酸、α - 酮戊二酸、琥珀酸、富马酸、苹果酸、半乳糖、甘露糖。这些代谢物与脂肪酸、氨基酸、柠檬酸循环和葡萄糖代谢相关。三个针刺组上述代谢物均有不同程度的调节（均P<0.05）。与提插补泻针法组和捻转补法组相比，温针组乳酸含量降低，琥珀酸、富马酸、苹果酸、半乳糖、甘露糖含量升高更明显。
温针治疗RA的特异性表现为对柠檬酸循环和葡萄糖代谢的调节作用。
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