Richter M, Cyranka U, Markwardt F
Institut für Pharmakologie und Toxikologie, Medizinischen Akademie Erfurt.
Pharmazie. 1990 Mar;45(3):200-3.
Human, rat and bovine fibrinogen were radioiodinated using a modified chloramine-T method under mild labelling conditions and with a satisfactory labelling efficiency. The 125I-human, rat and bovine fibrinogen preparations obtained met the requirements with respect to clottability, low content of 125Iodide as well as stability in vitro and in vivo. SDS-PAGE mobility demonstrated that the integrity of the fibrinogen molecule was not affected by the labelling procedure.
使用改良的氯胺-T法在温和的标记条件下对人、大鼠和牛纤维蛋白原进行放射性碘化，标记效率令人满意。所获得的125I-人、大鼠和牛纤维蛋白原制剂在可凝性、低碘化物含量以及体外和体内稳定性方面均符合要求。SDS-PAGE迁移率表明，标记过程未影响纤维蛋白原分子的完整性。
