Richter M, Cyranka U, Markwardt F
Institut für Pharmakologie und Toxikologie der Medizinischen Akademie Erfurt.
Pharmazie. 1989 Feb;44(2):147-9.
For the first time, the fibrinogen preparation according to Blombäck was used to purify rat fibrinogen. Intermediate as well as final products were compared with those of human fibrinogen. Yields of final products were about 25% of plasma fibrinogen. Thrombin clottability amounted to 96.5 +/- 1.3% for rat fibrinogen and 95.0 +/- 1.1% for human fibrinogen, respectively. The integrity and the characteristic differences in molecular mass of rat and human fibrinogen chains were demonstrated by means of SDS-PAGE.
首次使用根据布洛姆贝克方法制备的纤维蛋白原制剂来纯化大鼠纤维蛋白原。将中间产物和最终产物与人纤维蛋白原的产物进行了比较。最终产物的产量约为血浆纤维蛋白原的25%。大鼠纤维蛋白原和人纤维蛋白原的凝血酶可凝性分别为96.5±1.3%和95.0±1.1%。通过SDS-PAGE证实了大鼠和人纤维蛋白原链的完整性和分子量特征差异。
