Center for Stem Cell Biology and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Cell Metab. 2013 Jul 2;18(1):9-20. doi: 10.1016/j.cmet.2013.06.011.
Cre/LoxP has broad utility for studying the function, development, and oncogenic transformation of pancreatic cells in mice. Here we provide an overview of the Cre driver lines that are available for such studies. We discuss how variegated expression, transgene silencing, and recombination in undesired cell types have conspired to limit the performance of these lines, sometimes leading to serious experimental concerns. We also discuss preferred strategies for achieving high-fidelity driver lines and remind investigators of the continuing need for caution when interpreting results obtained from any Cre/LoxP-based experiment performed in mice.
Cre/LoxP 广泛用于研究小鼠胰腺细胞的功能、发育和致癌转化。本文提供了用于此类研究的 Cre 驱动线的概述。我们讨论了斑驳表达、转基因沉默和不期望的细胞类型中的重组如何共同限制了这些品系的性能，有时会导致严重的实验问题。我们还讨论了实现高保真驱动线的首选策略，并提醒研究人员在解释在小鼠中进行的任何基于 Cre/LoxP 的实验的结果时要继续保持谨慎。
