Department of Clinical Laboratory, Hubei Zhongshan Hospital, Wuhan, China.
Acta Biochim Biophys Sin (Shanghai). 2013 Sep;45(9):756-62. doi: 10.1093/abbs/gmt075. Epub 2013 Jul 3.
Recent studies have shown that microRNA-21 (miR-21) contributes to tumor resistance to chemotherapy. Interestingly, we have found that berberine could inhibit miR-21 expression in several cancer cell lines. In this study, we investigated whether berberine could modulate the sensitivity of ovarian cancer cells to cisplatin and explored the mechanism. The cisplatin-resistant SKOV3 cells that were incubated with berberine combined with cisplatin had a significantly lower survival than the cisplatin alone group and enhanced cisplatin-induced apoptosis. Berberine could inhibit miR-21 expression and function in ovarian cancer, as shown by an enhancement of its target PDCD4, an important tumor suppressor in ovarian cancer. The results suggested that berberine could modulate the sensitivity of cisplatin via regulating miR-21/PDCD4 axis in the ovarian cancer cells.
最近的研究表明，微小 RNA-21（miR-21）有助于肿瘤对化疗的耐药性。有趣的是，我们发现小檗碱可以抑制几种癌细胞系中的 miR-21 表达。在这项研究中，我们研究了小檗碱是否可以调节卵巢癌细胞对顺铂的敏感性，并探讨了其机制。用小檗碱联合顺铂孵育的顺铂耐药 SKOV3 细胞的存活率明显低于单独使用顺铂组，并增强了顺铂诱导的细胞凋亡。小檗碱可以抑制卵巢癌细胞中的 miR-21 表达和功能，表现为其靶基因 PDCD4 的增强，PDCD4 是卵巢癌中的一个重要肿瘤抑制因子。结果表明，小檗碱可以通过调节 miR-21/PDCD4 轴来调节顺铂在卵巢癌细胞中的敏感性。
