一种基于机器视觉的小动物视动反射测量系统。
A simple machine vision-driven system for measuring optokinetic reflex in small animals.
机构信息
Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, Toyama, Toyama 930-8555, Japan.
出版信息
J Physiol Sci. 2013 Sep;63(5):395-9. doi: 10.1007/s12576-013-0276-5. Epub 2013 Jul 4.
The optokinetic reflex (OKR) is useful to monitor the function of the visual and motor nervous systems. However, OKR measurement is not open to all because dedicated commercial equipment or detailed instructions for building in-house equipment is rarely offered. Here we describe the design of an easy-to-install/use yet reliable OKR measuring system including a computer program to visually locate the pupil and a mathematical procedure to estimate the pupil azimuth from the location data. The pupil locating program was created on a low-cost machine vision development platform, whose graphical user interface allows one to compose and operate the program without programming expertise. Our system located mouse pupils at a high success rate (90 %), estimated their azimuth precisely (94 %), and detected changes in OKR gain due to the pharmacological modulation of the cerebellar flocculi. The system would promote behavioral assessment in physiology, pharmacology, and genetics.
视动反射（OKR）可用于监测视觉和运动神经系统的功能。然而，由于很少有专门的商业设备或内部设备构建的详细说明，因此并非所有人都可以进行 OKR 测量。在这里，我们描述了一种易于安装/使用但可靠的 OKR 测量系统的设计，包括一个用于可视化定位瞳孔的计算机程序和一个从位置数据估计瞳孔方位的数学程序。瞳孔定位程序是在低成本机器视觉开发平台上创建的，其图形用户界面允许用户在无需编程专业知识的情况下组合和操作程序。我们的系统以高成功率（约 90%）定位了鼠标瞳孔，精确估计了它们的方位（约 94%），并检测到由于小脑绒球的药理学调制而导致的 OKR 增益变化。该系统将促进生理学、药理学和遗传学中的行为评估。
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