Ghorbanian Bahloul, Ravassi Aliasghar, Kordi Mohammad Reza, Hedayati Mahdi
Physical Education and Sports Sciences Department, Azarbaijan Shahid Madani University, Tabriz, IR Iran.
Int J Endocrinol Metab. 2013 Spring;11(2):76-81. doi: 10.5812/ijem.8178. Epub 2013 Apr 1.
Early obesity and its transfer to the adulthood, increases likelihood incidence of coronary artery disease (CAD). ATP-binding cassette transporter (ABCA1) as a member of the ABC transporters family plays a crucial role in reverse cholesterol transport and CAD prevention.
The current study aimed to investigate ABCA1 expression in lymphocytes, plasma apolipoprotein A-I and HDL-C in response to eight-week interval endurance rope training in overweight and obese boy adolescents.
Thirty students (17.3 ± 1.1 yr, 85.73 ± 11.68 kg and 28.41 ± 2.36 kg / m²) volunteered and were randomly assigned into training (n= 15) and control (n = 15) groups. Exercise protocol was interval endurance rope training (8 wk, 4 d/wk and 40 min/d). Cell hemolysis and sensitive Elisa method was used for Lymphocyte ABAC1 protein expression.t-test was employed.
The independent-samples T-Test results showed that after 8 weeks IERT, the levels of lymphocyte ABCA1 expression (P = 0/001) and VO2max(P = 0/001) significantly increased and plasma levels of TG (P = 0.017), TC (P = 0.001), LDL-c/HDL-c (P = 0.026),TC/HDL-c (P = 0.002) and measures of BF% (P = 0/015) and BMI (P = 0.042) as anthropometric indicators significantly decreased. Changes of other variables such as increase in ApoA-I, HDL-c and decrease in LDL-c, body weight, were not significant.
The findings of this study proved that eight-week interval endurance rope training can have positive effects on lymphocyte ABCA1 protein expression (as gatekeeper of reverse cholesterol process) and lipid profiles among overweight and obese boy adolescents.
早期肥胖及其延续至成年期会增加冠状动脉疾病（CAD）的发病可能性。ATP结合盒转运蛋白（ABCA1）作为ABC转运蛋白家族的一员，在逆向胆固醇转运和预防CAD中起关键作用。
本研究旨在调查超重和肥胖男青少年在进行为期八周的间歇性耐力跳绳训练后，淋巴细胞中ABCA1的表达、血浆载脂蛋白A-I和高密度脂蛋白胆固醇（HDL-C）的变化。
30名学生（年龄17.3±1.1岁，体重85.73±11.68千克，体重指数28.41±2.36千克/平方米）自愿参与并被随机分为训练组（n = 15）和对照组（n = 15）。运动方案为间歇性耐力跳绳训练（8周，每周4天，每天40分钟）。采用细胞溶血和灵敏酶联免疫吸附测定法检测淋巴细胞ABAC1蛋白表达。使用t检验。
独立样本T检验结果显示，八周间歇性耐力跳绳训练后，淋巴细胞ABCA1表达水平（P = 0.001）和最大摄氧量（P = 0.001）显著升高，血浆甘油三酯（TG）水平（P = 0.017）、总胆固醇（TC）水平（P = 0.001）、低密度脂蛋白胆固醇/高密度脂蛋白胆固醇（LDL-c/HDL-c，P = 0.026）、总胆固醇/高密度脂蛋白胆固醇（TC/HDL-c，P = 0.002）以及作为人体测量指标的体脂百分比（BF%，P = 0.015）和体重指数（BMI，P = 0.042）显著降低。其他变量的变化，如载脂蛋白A-I升高、HDL-C升高以及低密度脂蛋白胆固醇、体重降低，均不显著。
本研究结果证明，为期八周的间歇性耐力跳绳训练可对超重和肥胖男青少年淋巴细胞ABCA1蛋白表达（作为逆向胆固醇过程的守门人）和血脂状况产生积极影响。
