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新生仔猪肌纤维的组织化学发育

Histochemical development of myofibres in neonatal piglets.

作者信息

Swatland H J

出版信息

Res Vet Sci. 1975 May;18(3):253-7.

PMID:238264
Abstract

The periodic acid-Schiff (PAS) reaction and tests for glycogen phosphorylase, oxidative enzymes and acid-stable and alkali-stable adenosine triphophatase (ATPase) were used to determine the degree of histochemical differentiation between myofibres of sartorius muscles from neonatal piglets. Within 24 h of birth, the ratio of myofibres with acid-stable ATPase to those with acid-labile ATPase was 1:21-0. By 10 days the ratio had changed to 1:5-2. Mean minimum myofibre diameters (all histochemical types combined) increased steadily after birth although diameters of myofibres with acid-stable ATPase showed no increase until 6 days. By 10 days, consistent differentiation was observed with the alkali-stable ATPase and glycogen phosphorylase reactions but not with the PAS reaction. During the 10 day neonatal period, all types of myofibres contained large or moderate numbers of mitochondria.

摘要

采用过碘酸 - 希夫(PAS)反应以及糖原磷酸化酶、氧化酶和酸稳定及碱稳定三磷酸腺苷酶(ATP酶)检测,来确定新生仔猪缝匠肌肌纤维之间的组织化学分化程度。出生后24小时内,酸稳定ATP酶阳性肌纤维与酸不稳定ATP酶阳性肌纤维的比例为1:21 - 0。到10日龄时,该比例变为1:5 - 2。出生后,所有组织化学类型合并计算的平均最小肌纤维直径稳步增加,不过酸稳定ATP酶阳性肌纤维的直径直到6日龄才开始增加。到10日龄时,碱稳定ATP酶和糖原磷酸化酶反应呈现出一致的分化,但PAS反应未出现。在新生期的10天里,所有类型的肌纤维都含有大量或中等数量的线粒体。

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4
Allometric radial growth in muscle, comparing fibres with strong and with weak adenosine triphosphatase activity.肌肉中的异速径向生长,比较具有强和弱三磷酸腺苷酶活性的纤维。
J Anat. 1979 Oct;129(Pt 3):591-6.
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6
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Histochem J. 1975 Sep;7(5):459-69. doi: 10.1007/BF01003883.