Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, SP, Brasil.
Mem Inst Oswaldo Cruz. 2013 Jun;108(4):529-31. doi: 10.1590/S0074-02762013000400023.
Culex quinquefasciatus mosquitoes have been successfully genetically modified only once, despite the efforts of several laboratories to transform and establish a stable strain. We have developed a transient gene expression method, in Culex, that delivers plasmid DNA directly to the mosquito haemolymph and additional tissues. We were able to express DsRed2 fluorescent protein in adult Cx. quinquefasciatus mosquitoes by injecting plasmids directly into their thorax. The expression of DsRed2 in adult Cx. quinquefasciatus mosquitoes is an important stepping stone to genetic transformation and the potential use of new control strategies and genetic interactions.
尽管有几个实验室努力进行转化并建立稳定株，但仅有一次成功对致倦库蚊进行了基因修饰。我们开发了一种在致倦库蚊中的瞬时基因表达方法，可将质粒 DNA 直接递送至蚊血淋巴和其他组织。我们通过直接向其胸部注射质粒，使 DsRed2 荧光蛋白在成年致倦库蚊中表达。在成年致倦库蚊中表达 DsRed2 是基因转化的重要垫脚石，也是潜在的新控制策略和遗传相互作用的应用的基础。
