Coelho Junior Aloisio, Parra José R P
Departamento de Entomologia e Acarologia, Escola Superior de Agricultura Luiz de Queiroz/ESALQ, Universidade de São Paulo/USP, Piracicaba, SP, Brasil.
An Acad Bras Cienc. 2013 Apr-Jun;85(2):823-31. doi: 10.1590/S0001-37652013000200021.
Eggs of Anagasta kuehniella (Zeller 1879) are widely used for mass rearing of Trichogramma spp. and other parasitoids and predators, largely commercialized in many countries. The aim of this study is to evaluate the effect of carbon dioxide (CO2) originated from larval metabolism on the biological parameters of A. kuehniella. For that purpose, we assess the production of carbon dioxide (CO2) per rearing tray of A. kuehniella and the effect of CO2 on the viability of egg-to-adult period and oviposition of A. kuehniella. Results allow to estimate that a rearing tray, containing 10,000 larvae between the 4th and 5th instars, produces an average of 30.67 mL of CO2 per hour. The highest egg production of A. kuehniella was obtained when the larvae were kept in rooms with lower concentration of CO2 (1,200 parts per million - ppm), producing 23% more eggs than in rooms with higher CO2 concentrations. In rooms with high density of trays (70 trays/room), CO2 concentration exceeded 4,400 ppm. The viability of the egg-to-adult period was not influenced by carbon dioxide.
地中海粉螟（Anagasta kuehniella，泽勒，1879年）的卵被广泛用于大量饲养赤眼蜂属（Trichogramma spp.）及其他寄生性和捕食性天敌昆虫，在许多国家已大量商业化。本研究的目的是评估幼虫代谢产生的二氧化碳（CO₂）对地中海粉螟生物学参数的影响。为此，我们评估了每个饲养托盘的地中海粉螟产生二氧化碳（CO₂）的量，以及CO₂对地中海粉螟卵到成虫期的存活率和产卵的影响。结果表明，一个饲养托盘在4龄和5龄期饲养10,000只幼虫时，每小时平均产生30.67毫升CO₂。当地中海粉螟幼虫饲养在CO₂浓度较低（百万分之1200 - ppm）的房间时，产卵量最高，比CO₂浓度较高的房间多产23%的卵。在托盘密度高的房间（70个托盘/房间），CO₂浓度超过4400 ppm。卵到成虫期的存活率不受二氧化碳影响。
