LC Resources, Walnut Creek, CA, USA.
J Chromatogr A. 2013 Aug 9;1302:45-54. doi: 10.1016/j.chroma.2013.05.082. Epub 2013 Jun 10.
Several different conditions can be varied to improve selectivity for reversed-phase chromatography (RPC). A reexamination of literature data suggests that changes in selectivity due to a change of column or mobile phase pH are largely replicated by changes in temperature or mobile phase composition (concentrations of acetonitrile and/or methanol). This suggests a reconsideration of the role of mobile phase pH and the column during method development.
几种不同的条件可以改变以提高反相色谱（RPC）的选择性。对文献数据的重新检查表明，由于柱或流动相 pH 值的变化而导致的选择性变化在很大程度上可以通过温度或流动相组成（乙腈和/或甲醇的浓度）的变化来复制。这表明在方法开发过程中需要重新考虑流动相 pH 值和柱的作用。
