Fueangfung Suntara, Yuan Yinan, Fang Shiyue
a Department of Chemistry , Michigan Technological University , Houghton , Michigan , USA.
Nucleosides Nucleotides Nucleic Acids. 2014;33(7):481-8. doi: 10.1080/15257770.2014.891740.
Denaturing reversed-phase (RP) high performance liquid chromatography (HPLC) is usually achieved by elevating column temperature. In this article, an alternative method involving using a mobile phase that contains urea and performing HPLC at room temperature is described. The efficacy of the new method was demonstrated by analyzing a 61-mer oligodeoxynucleotide (ODN) and double-stranded (ds) ODNs. The multiple peaks of the 61-mer ODN under normal conditions merged into one under the denaturing conditions. The broad single peaks of dsODNs under normal conditions were split into two sharp peaks.
变性反相（RP）高效液相色谱法（HPLC）通常通过升高柱温来实现。在本文中，描述了一种替代方法，即使用含有尿素的流动相并在室温下进行HPLC。通过分析一个61聚体寡脱氧核苷酸（ODN）和双链（ds）ODN证明了该新方法的有效性。正常条件下61聚体ODN的多个峰在变性条件下合并为一个峰。正常条件下dsODN的宽单峰分裂为两个尖锐峰。
