Instituto de Engenharia Biomédica (INEB), Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; Faculdade de Engenharia, Universidade do Porto, Porto, Portugal.
Acta Biomater. 2013 Nov;9(11):8885-93. doi: 10.1016/j.actbio.2013.06.042. Epub 2013 Jul 4.
Helicobacter pylori colonizes the gastric mucosa of half of the worlds population and persistent infection is related with an increase in the risk of gastric cancer. Adhesion of H. pylori to the gastric epithelium, which is essential for infection, is mediated by bacterial adhesin proteins that recognize specific glycan structures (Gly-R) expressed in the gastric mucosa. The blood group antigen binding adhesin (BabA) recognizes difucosylated antigens such as Lewis B (Leb), while the sialic acid binding adhesin (SabA) recognizes sialylated glycoproteins and glycolipids, such as sialyl-Lewis x (sLex). This work aimed to investigate whether these Gly-Rs (Leb and sLex) can attract and specifically bind H. pylori after immobilization on synthetic surfaces (self-assembled monolayers (SAMs) of alkanethiols on gold). Functional bacterial adhesion assays for (Gly-R)-SAMs were performed using H. pylori strains with different adhesin protein profiles. The results demonstrate that H. pylori binding to surfaces occurs via interaction between its adhesins and cognate (Gly-R)-SAMs and bound H. pylori maintains its characteristic rod-shaped morphology only during conditions of specific adhesin-glycan binding. These results offer new insights into innovative strategies against H. pylori infection based on the scavenging of bacteria from the stomach using specific H. pylori chelating biomaterials.
幽门螺杆菌定植于世界上一半人口的胃黏膜，持续性感染与胃癌风险增加相关。幽门螺杆菌对胃上皮细胞的黏附是感染的必要条件，这一过程由细菌黏附蛋白介导，这些蛋白可以识别胃黏膜中表达的特定糖链结构（Gly-R）。血型抗原结合黏附素（BabA）识别双岩藻糖基化抗原，如Lewis B（Leb），而唾液酸结合黏附素（SabA）识别唾液酸化糖蛋白和糖脂，如唾液酸化-Lewis x（sLex）。本研究旨在探讨这些 Gly-R（Leb 和 sLex）在固定于合成表面（金上的烷硫醇自组装单层（SAMs））后是否可以吸引并特异性结合幽门螺杆菌。使用具有不同黏附蛋白谱的幽门螺杆菌菌株进行了（Gly-R）-SAM 的功能性细菌黏附测定。结果表明，幽门螺杆菌与表面的结合是通过其黏附素与相应的（Gly-R）-SAM 之间的相互作用实现的，并且只有在特定黏附素-聚糖结合的条件下，结合的幽门螺杆菌才能保持其特征性的杆状形态。这些结果为基于使用特定的幽门螺杆菌螯合生物材料从胃中清除细菌来对抗幽门螺杆菌感染的创新策略提供了新的见解。
