Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
Respir Physiol Neurobiol. 2013 Oct 1;189(1):1-9. doi: 10.1016/j.resp.2013.06.021. Epub 2013 Jul 5.
We investigated the mediator mechanisms involved in the sensory transduction of pulmonary reactive oxygen species (ROS) by vagal lung C-fibers in anesthetized rats. Airway challenge of aerosolized H₂O₂ (0.4%) stimulated these afferent fibers. The H₂O₂-induced responses were reduced by a cyclooxygenase inhibitor or ATP scavengers and also attenuated by an antagonist of TRPV1, TRPA1 or P2X receptors. The suppressive effect of the cyclooxygenase inhibitor was not affected by a combined treatment with the TRPV1 or TRPA1 antagonist, but was amplified by a combined treatment with the P2X antagonists. The suppressive effect of ATP scavengers was not affected by a combined treatment with the P2X antagonist, but was amplified by a combined treatment with the TRPV1 or TRPA1 antagonist. Thus, the actions of cyclooxygenase metabolites are mediated through the functioning of the TRPV1 and TRPA1 receptors, whereas the action of ATP is mediated through the functioning of P2X receptors.
我们研究了在麻醉大鼠中，迷走神经肺 C 纤维对肺部活性氧（ROS）感觉转导所涉及的中介机制。雾化 H₂O₂（0.4%）的气道挑战刺激了这些传入纤维。环氧合酶抑制剂或 ATP 清除剂可减少 H₂O₂诱导的反应，而 TRPV1、TRPA1 或 P2X 受体拮抗剂也可减弱其反应。环氧合酶抑制剂的抑制作用不受 TRPV1 或 TRPA1 拮抗剂联合处理的影响，但可被 P2X 拮抗剂联合处理放大。ATP 清除剂的抑制作用不受 P2X 拮抗剂联合处理的影响，但可被 TRPV1 或 TRPA1 拮抗剂联合处理放大。因此，环氧合酶代谢物的作用是通过 TRPV1 和 TRPA1 受体的功能来介导的，而 ATP 的作用是通过 P2X 受体的功能来介导的。
