Hedayatkhah Abolghasem, Motamedi Hossein, Najafzadeh Varzi Hossein, Ghezelbash Gholamreza, Amopour Bahnamiry Mostafa, Karimi Keikhosro
Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz 61357-43135, Iran.
Biosci Biotechnol Biochem. 2013;77(7):1379-83. doi: 10.1271/bbb.120867. Epub 2013 Jul 7.
Sugarcane bagasse was pretreated by soaking it in aqueous ammonia (SAA) and methanolic aqueous ammonia (SMAA) at 70 °C for 12 h. Then the pretreated as well as untreated bagasse was subjected to enzymatic hydrolysis at 50 °C for 72 h by 15 FPU cellulase and 30 CBU cellobiase per g of substrate. The hydrolysis of SAA-pretreated bagasse with a solid to liquid (S:L) ratio of 1:10 resulted in 95.9% of the maximum theoretical yield. The production yield for SMAA at an S:L ratio of 1:6 with 15% methanol was 88.6%, while it was only 21.3% for the untreated bagasse. Ethanol production by simultaneous saccharification and fermentation was conducted at 37 °C for 72 h. The results revealed that the ethanol production yield was improved from 12.7% for the untreated bagasse to 92.45% and 90.8% for the SAA and the SMAA pretreated bagasse, respectively. The compositional and chemical structural analysis suggested that lignin removal and crystallinity reduction were responsible for the hydrolysis and SSF improvements.
甘蔗渣在70℃下于氨水（SAA）和甲醇氨水（SMAA）中浸泡12小时进行预处理。然后，将预处理过的以及未处理的甘蔗渣在50℃下，每克底物使用15 FPU纤维素酶和30 CBU纤维二糖酶进行72小时的酶水解。SAA预处理的甘蔗渣在固液比（S:L）为1:10时进行水解，得到了最大理论产率的95.9%。SMAA在固液比为1:6且含有15%甲醇时的产率为88.6%，而未处理的甘蔗渣产率仅为21.3%。通过同步糖化发酵在37℃下进行72小时的乙醇生产。结果表明，乙醇产率从未处理甘蔗渣的12.7%分别提高到了SAA预处理甘蔗渣的92.45%和SMAA预处理甘蔗渣的90.8%。成分和化学结构分析表明，木质素的去除和结晶度的降低是水解和同步糖化发酵改善的原因。
