某些N端肽可抑制分离的线粒体对成熟天冬氨酸转氨酶的摄取。

Certain N-terminal peptides inhibit uptake of mature aspartate aminotransferase by isolated mitochondria.

作者信息

Barile M, Giannattasio S, Marra E, Passarella S, Pucci P, Sannia G, Quagliariello E

机构信息

Dipartimento di Biochimica e Biologia Molecolare, Università degli Studi, Bari-Italy.

出版信息

Biochem Biophys Res Commun. 1990 Jul 31;170(2):609-15. doi: 10.1016/0006-291x(90)92135-m.

DOI:10.1016/0006-291x(90)92135-m

Abstract

To gain insight into the uptake of mature aspartate aminotransferase by isolated mitochondria, the capability of certain cyanogen bromide peptides from mature beef heart mitochondrial aspartate aminotransferase to inhibit enzyme uptake was kinetically tested. N-terminal peptides (1-9 and 10-31) proved to inhibit the rate of aspartate aminotransferase uptake respectively in purely competitive and non-competitive ways, whereas other peptides distal from the N-terminus (203-217, 321-327 and 328-353) were found to be completely ineffective.

摘要

为深入了解分离的线粒体对成熟天冬氨酸氨基转移酶的摄取情况，对来自成熟牛心线粒体天冬氨酸氨基转移酶的某些溴化氰肽抑制酶摄取的能力进行了动力学测试。N端肽（1-9和10-31）分别以纯竞争性和非竞争性方式抑制天冬氨酸氨基转移酶的摄取速率，而其他远离N端的肽（203-217、321-327和328-353）则完全无效。

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