Lv Xiao-Guang, Lei Xiao-Fei, Ji Meng-Yao, Guo Xu-Feng, Wang Jing, Dong Wei-Guo
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
Oncol Lett. 2013 Jun;5(6):1844-1848. doi: 10.3892/ol.2013.1271. Epub 2013 Mar 26.
Ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) is a postsynaptic density-95/disc-large/zonula occludens-1 (PDZ) homologous domain-containing protein that is involved in cell signaling. EBP50 regulates cell apoptosis, proliferation and invasion. In the present study, the prognostic impact factor of EBP50 expression was evaluated using a quantum dot (QD)-based assay and immunohistochemistry (IHC). The EBP50 protein expression in gastric cancer (GC) tissues was evaluated using IHC and QD-IHC. The study included 101 patients with GC (29 females and 72 males, aged 24-81 years), diagnosed and treated at the General Surgery Department of Renmin Hospital of Wuhan University (Wuhan, China) between 2000 and 2005. The survival rate was calculated using the Kaplan-Meier method and log-rank tests. IHC and QD analyses of 101 GC tissue specimens revealed that EBP50-positive tumor cells were frequently present in GC. Increased EBP50 immunostaining was observed in 63 specimens (62.4%). The EBP50 expression levels were correlated with increased tumor size and the male gender. EBP50 was well distributed in the cytoplasm and nuclei of the GC cells. However, EBP50 protein expression exhibited no correlation with age, differentiation, stage or lymph node metastasis. There were no associations between the expression of EBP50 and the mean survival rates (IHC, 50.5 vs. 58.1 months, P>0.05; QD, 55.4 vs. 63.2 months, P>0.05). These findings suggest that EBP50 protein expression is not correlated with the prognosis of patients with GC. QD-IHC and IHC have similar advantages for the detection of EBP50 protein expression.
埃兹蛋白-根蛋白-膜突蛋白结合磷蛋白50（EBP50）是一种含有突触后致密蛋白95/盘状大蛋白/紧密连接蛋白1（PDZ）同源结构域的蛋白，参与细胞信号传导。EBP50调节细胞凋亡、增殖和侵袭。在本研究中，使用基于量子点（QD）的检测方法和免疫组织化学（IHC）评估了EBP50表达的预后影响因素。采用免疫组织化学和量子点免疫组织化学方法评估胃癌（GC）组织中EBP50蛋白的表达。该研究纳入了101例GC患者（29例女性和72例男性，年龄24 - 81岁），这些患者于2000年至2005年在武汉大学人民医院（中国武汉）普通外科确诊并接受治疗。采用Kaplan-Meier法和对数秩检验计算生存率。对101份GC组织标本进行免疫组织化学和量子点分析发现，GC中经常存在EBP50阳性肿瘤细胞。在63份标本（62.4%）中观察到EBP50免疫染色增加。EBP50表达水平与肿瘤大小增加和男性性别相关。EBP50在GC细胞的细胞质和细胞核中分布良好。然而，EBP50蛋白表达与年龄、分化程度、分期或淋巴结转移无关。EBP50的表达与平均生存率之间无相关性（免疫组织化学，50.5个月对58.1个月，P>0.05；量子点检测，55.4个月对63.2个月，P>0.05）。这些发现表明，EBP50蛋白表达与GC患者的预后无关。量子点免疫组织化学和免疫组织化学在检测EBP50蛋白表达方面具有相似的优势。
