van Leeuwen A M, de Lange G G
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, University of Amsterdam.
Exp Clin Immunogenet. 1990;7(3):141-7.
With murine monoclonal antibodies specific for the human IgG1 allotypes G1m(z), G1m(a), G1m(f) and the IgG1 subclass, sandwich enzyme-linked immunosorbent assays (ELISAs) were developed to determine whether different allotypic determinants are located on the same molecule. With the conventional haemagglutination-inhibition test, it is only possible to determine the phenotype. With this ELISA system, it is now possible to deduce the G1m genotype.
利用针对人IgG1同种异型G1m(z)、G1m(a)、G1m(f)以及IgG1亚类的鼠单克隆抗体，开发了夹心酶联免疫吸附测定（ELISA），以确定不同的同种异型决定簇是否位于同一分子上。使用传统的血凝抑制试验，只能确定表型。而借助这种ELISA系统，现在可以推断出G1m基因型。
