De Lange G G, van Leeuwen A M
Central Laboratory, The Netherlands Red Cross Blood Transfusion Service, University of Amsterdam.
Immunology. 1991 Nov;74(3):559-60.
Murine monoclonal anti-human Ig allotype antibodies have been described for a limited number of specificities. Now the first monoclonal anti-G1m(x) antibody has been produced by fusion of lymph gland cells of a mouse injected with G1m(zax)-positive myeloma proteins with cells of the myeloma cell line SP2/0. Several limiting dilutions resulted in one antibody-producing hybridoma, clone 3A12. The specificity of monoclonal antibody (mAb) 3A12 in the direct haemagglutination assay, as well as in the haemagglutination-inhibition (HAI) assay, is described. Neither cross-reactivity nor nonspecific inhibition with G1m(x)-negative Ig was seen. Typing for the G1m(x) allotype with mAb 3A12 gave results which are identical to those obtained with the conventional human antisera. With the production of this mAb, it now is possible to discriminate between the four main G1m alleles not only by HAI assay but also by other assay systems, of which the preliminary results are promising.
已描述了针对有限数量特异性的鼠源单克隆抗人Ig同种异型抗体。现在，通过将注射了G1m(zax)阳性骨髓瘤蛋白的小鼠的淋巴结细胞与骨髓瘤细胞系SP2/0的细胞融合，产生了第一种单克隆抗G1m(x)抗体。经过几次有限稀释后得到了一个产生抗体的杂交瘤，即克隆3A12。描述了单克隆抗体（mAb）3A12在直接血凝试验以及血凝抑制（HAI）试验中的特异性。未观察到与G1m(x)阴性Ig的交叉反应或非特异性抑制。用mAb 3A12对G1m(x)同种异型进行分型，所得结果与用传统人抗血清获得的结果相同。随着这种mAb的产生，现在不仅可以通过HAI试验，还可以通过其他检测系统区分四种主要的G1m等位基因，初步结果很有前景。
