Fernandes A F A, Alvarenga É R, Oliveira D A A, Aleixo C G, Prado S A, Luz R K, Sarmento N L A F, Teixeira E A, Luz M R, Turra E M
Laboratório de Aquacultura (LAQUA), Escola de Veterinária da Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Reprod Domest Anim. 2013 Dec;48(6):1049-55. doi: 10.1111/rda.12212. Epub 2013 Jul 9.
Only a few studies have described hormonal treatments for induction of synchronicity and gamete collection in Nile tilapia (Oreochromis niloticus), both important for assortative matings in breeding programmes and essential for polyploidy technologies. In this study, we compared the effectiveness of carp pituitary extract (CPE), Nile tilapia pituitary extract (TPE), human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone (GnRH) protocols on the induction of spawning and egg production in Nile tilapia. Among the hormonal treatments analysed, only hCG was effective for producing viable gametes for in vitro fertilization. To verify the viability of this hormonal treatment, hCG was tested using different doses (1000, 2000, 3000, 4000 and 5000 IU/kg) in a large number of females (208 animals) from two Nile tilapia lines. The results indicated that hCG doses between 1000 and 5000 IU/kg could be used to induce final oocyte maturation in Nile tilapia with collection of stripped oocytes. This is the first study to report differential reproductive responses to hormonal treatment between tilapia lines: line 1 was more efficient at producing eggs and post-hatching larvae after hCG induction than line 2. In conclusion, we demonstrated that the hCG protocol may be applied on a large scale to induce final oocyte maturation in Nile tilapia. The development of a protocol for in vitro fertilization in Nile tilapia may aid in breeding programmes and biotechnological assays for the development of genetically modified lines of Nile tilapia.
仅有少数研究描述了用于诱导尼罗罗非鱼（Oreochromis niloticus）同步性和采集配子的激素处理方法，这两者对于育种计划中的配对交配都很重要，并且是多倍体技术所必需的。在本研究中，我们比较了鲤鱼垂体提取物（CPE）、尼罗罗非鱼垂体提取物（TPE）、人绒毛膜促性腺激素（hCG）和促性腺激素释放激素（GnRH）方案对尼罗罗非鱼产卵和产蛋的诱导效果。在所分析的激素处理中，只有hCG能有效产生用于体外受精的有活力配子。为了验证这种激素处理的可行性，我们在来自两个尼罗罗非鱼品系的大量雌性（208只动物）中使用不同剂量（1000、2000、3000、4000和5000 IU/kg）的hCG进行了测试。结果表明，1000至5000 IU/kg的hCG剂量可用于诱导尼罗罗非鱼最终卵母细胞成熟并采集剥离的卵母细胞。这是第一项报道罗非鱼品系对激素处理有不同繁殖反应的研究：品系1在hCG诱导后产生卵子和孵化后幼体的效率高于品系2。总之，我们证明了hCG方案可大规模应用于诱导尼罗罗非鱼最终卵母细胞成熟。尼罗罗非鱼体外受精方案的开发可能有助于育种计划以及尼罗罗非鱼转基因品系开发的生物技术检测。
