CIIMAR/CIMAR - Interdisciplinary Centre of Marine and Environmental Research, Laboratory of Environmental Toxicology, University of Porto, Rua dos Bragas, 289, 4050-123, Porto, Portugal; ICBAS/UP - Institute of Biomedical Sciences Abel Salazar, University of Porto, Largo Professor Abel Salazar, 2, 4099-003, Porto, Portugal.
CIIMAR/CIMAR - Interdisciplinary Centre of Marine and Environmental Research, Laboratory of Environmental Toxicology, University of Porto, Rua dos Bragas, 289, 4050-123, Porto, Portugal.
Environ Toxicol Pharmacol. 2013 Sep;36(2):611-625. doi: 10.1016/j.etap.2013.05.017. Epub 2013 Jun 10.
The protein levels and tissue distribution patterns of P-glycoprotein (Pgp) and cytochrome P450 (CYP1A) were investigated in Nile tilapia (Oreochromis niloticus) after waterborne exposure to different benzo(a)pyrene (BaP) concentrations, using immunochemical approaches. The Pgp mammalian monoclonal antibody (mAb) C219 cross reacted with a ∼170kDa protein, almost exclusively localized to the bile canaliculi, while probing with the Pgp mammalian mAb C494, resulted in a positive reaction in liver, gills and intestine of Nile tilapia and covered a wider set of cell types. Levels of Pgp expression were not altered after in vivo exposure to BaP. CYP1A, detected with the mAb C10-7, reacted positively in liver, gills and intestine and followed a BaP dose-dependent fold induction. Taken together, these results indicate that CYP1A is involved in BaP metabolism in liver, gills and intestine, however, further studies are needed to elucidate the possible interaction of the efflux protein Pgp with BaP and/or its metabolites.
采用免疫化学方法研究了尼罗罗非鱼（Oreochromis niloticus）在经水暴露于不同浓度苯并（a）芘（BaP）后，P-糖蛋白（Pgp）和细胞色素 P450（CYP1A）的蛋白水平和组织分布模式。Pgp 哺乳动物单克隆抗体（mAb）C219 与大约 170kDa 的蛋白质发生交叉反应，该蛋白质几乎仅定位于胆小管，而用 Pgp 哺乳动物 mAb C494 探测，则在尼罗罗非鱼的肝脏、鳃和肠中产生阳性反应，并覆盖了更广泛的细胞类型。体内暴露于 BaP 后，Pgp 的表达水平没有改变。用 mAb C10-7 检测到的 CYP1A 在肝脏、鳃和肠中呈阳性反应，并随 BaP 剂量呈倍数诱导。总之，这些结果表明 CYP1A 参与了肝脏、鳃和肠中 BaP 的代谢，然而，需要进一步的研究来阐明外排蛋白 Pgp 与 BaP 和/或其代谢物之间可能的相互作用。
