CIIMAR/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, Laboratory of Environmental Toxicology, University of Porto, Rua dos Bragas, 289, 4050-123, Porto, Portugal.
Comp Biochem Physiol C Toxicol Pharmacol. 2012 Apr;155(3):469-82. doi: 10.1016/j.cbpc.2011.12.004. Epub 2011 Dec 23.
The aim of this study was to evaluate the response of ABC transporters, CYP1A and class alpha (α) GST genes, upon water and dietary exposures to benzo(a)pyrene (BaP) in Oreochromis niloticus. Partial mRNA sequences of ABC transporters (ABCB1b, ABCB11, ABCC1, ABCC2 and ABCG2) were identified, and their tissue distribution patterns evaluated in liver, gill and intestine, showing similarities with other fish and mammals. After 14 days of water exposure to BaP, ABC transporters mRNA expression was up-regulated, namely ABCC2 in gill (up to 16-fold) and ABCG2 in liver (up to 2-fold) and proximal intestine (up to 7-fold). CYP1A mRNA expression was up-regulated in water exposed animals, with maximum fold inductions of 5, 35 and 155, respectively in liver, gill and proximal intestine. After dietary exposure, intestinal CYP1A mRNA showed a 13-fold increase in exposed animals. No significant changes were seen in ABCB1b, ABCC1 and GSTα mRNA expression after both routes of exposure to BaP. In conclusion, this study has shown that transcriptional expression of some ABC transporters and CYP1A respond to the presence of BaP, indicating a possible involvement and cooperation in the detoxification process in Nile tilapia.
本研究旨在评估 ABC 转运体、CYP1A 和α 类 GST 基因在尼罗罗非鱼（Oreochromis niloticus）水相和食相暴露于苯并(a)芘（BaP）时的反应。鉴定了 ABC 转运体（ABCB1b、ABCB11、ABCC1、ABCC2 和 ABCG2）的部分 mRNA 序列，并评估了它们在肝脏、鳃和肠道中的组织分布模式，与其他鱼类和哺乳动物具有相似性。在水相暴露于 BaP 14 天后，ABCC2 在鳃（高达 16 倍）和 ABCG2 在肝脏（高达 2 倍）和近端肠道（高达 7 倍）中的 mRNA 表达上调。水暴露动物的 CYP1A mRNA 表达上调，肝脏、鳃和近端肠道的最大诱导倍数分别为 5、35 和 155。经食相暴露后，暴露动物的肠道 CYP1A mRNA 增加了 13 倍。在 BaP 的两种暴露途径后，ABCB1b、ABCC1 和 GSTα mRNA 表达均无明显变化。综上所述，本研究表明，一些 ABC 转运体和 CYP1A 的转录表达对 BaP 的存在有反应，表明它们可能参与并合作参与了尼罗罗非鱼的解毒过程。
