National Center for Nanoscience and Technology, No.11 ZhongGuanCun BeiYiTiao, 100190 Beijing, PR China.
Lab Chip. 2013 Sep 7;13(17):3347-50. doi: 10.1039/c3lc50632k. Epub 2013 Jul 8.
We present a microfluidic synthesizer made entirely of Teflon material for solid phase peptide synthesis (SPPS). Solvent-resistant perfluoroalkoxy (PFA) was used to construct chip-sized devices featuring multiple tri-layer pneumatic microvalves. Using these devices, model peptides were automatically synthesized and cleaved in situ in a continuous-flow manner. The total coupling and cleavage time was significantly reduced compared to conventional bulk reactors. The synthesis of a decapeptide, for instance, took less than 6 h using our device while it usually takes more than three days using conventional reactors.
我们展示了一种完全由 Teflon 材料制成的用于固相肽合成 (SPPS) 的微流合成器。耐溶剂的全氟烷氧基 (PFA) 用于构建具有多个三层气动微阀的芯片尺寸设备。使用这些设备，模型肽以连续流动的方式自动合成并原位切割。与传统的批量反应器相比，总偶联和切割时间显著缩短。例如，使用我们的设备合成一个十肽不到 6 小时，而使用传统的反应器通常需要三天以上。
