Institute of Bioprocess and Biosystems Engineering, Technische Universität Hamburg-Harburg, Denickestr. 15, 21073, Hamburg, Germany,
Adv Biochem Eng Biotechnol. 2013;137:153-84. doi: 10.1007/10_2013_232.
: In vitro multienzymatic bioreaction systems are attracting increasing attention for the development of bioproduction systems. The de-coupling of the biocatalytic pathway from the cellular machinery aimed at growth and survival allows achievement of high product yields and thus reduces byproduct or waste generation. Additionally, the use of several enzymes allows the realization of much more complex synthetic schemes, thus expanding the chemical diversity of synthetic compounds with new chemical properties or bioactivities. This chapter provides a survey of in vitro multienzymatic bioreaction systems used for biosynthesis, discusses process design aspects for the technical realization of multienzymatic bioreactions, highlights advantages and recent developments in using multienzyme microreactors, and finally reviews examples and strategies of the co-immobilization of multienzymes in nano/microsized materials.
体外多酶生物反应系统正越来越受到关注，用于开发生物生产系统。将生物催化途径与生长和存活的细胞机制解耦，可实现高产物产率，从而减少副产物或废物的产生。此外，使用多种酶可以实现更复杂的合成方案，从而扩大具有新化学性质或生物活性的合成化合物的化学多样性。本章介绍了用于生物合成的体外多酶生物反应系统，讨论了多酶生物反应技术实现的过程设计方面，强调了使用多酶微反应器的优势和最新进展，最后综述了多酶共固定在纳米/微米级材料中的实例和策略。
