Jackson C W, Steward S A, Chenaille P J, Ashmun R A, McDonald T P
Department of Hematology-Oncology, St. Jude Children's Research Hospital, Memphis 38101.
Blood. 1990 Aug 15;76(4):690-6.
The modal DNA content of normal marrow megakaryocytes from species so far examined usually has been reported to be 16N. In this report we describe an exception in the C3H mouse whose megakaryocytes have a modal DNA content of 32N. Female C3H/HEN mice had an average DNA content distribution of 14% 8N, 37% 16N, 43% 32N, and 6% 64N. Male C3H/HEN mice had somewhat higher proportions of 32N and 64N megakaryocytes (average DNA content distribution of 12% 8N, 29% 16N, 47% 32N, and 12% 64N) than females. All 11 other mouse strains examined had 16N as the modal megakaryocyte DNA content, although the proportions in the various polyploid DNA classes showed some strain variation. Megakaryocyte size was similar among all 12 strains evaluated, and mean platelet volume (MPV) of C3H/HEN mice differed from only 1 of the other 4 strains analyzed. Platelet counts of C3H/HEN mice were similar to those of six, and slightly but significantly lower than those of five other mouse strains examined. Compared with megakaryocyte concentrations of other mouse strains studied, that of C3H/HEN mice was similar to seven, somewhat higher than one, and slightly lower than three strains. Offspring from reciprocal matings of C57BL/6 and C3H/HEN mice had megakaryocyte DNA distributions intermediate between those of the parent strains, suggesting that a higher gene dosage of some component is responsible for the right-shifted megakaryocyte DNA content distribution phenotype of C3H mice. The proportions of 32N and 64N megakaryocytes increased in C3H/HEN mice in response to acute thrombocytopenia, as did those of CBA/CAJ mice used as a comparative strain. In summary, megakaryocytes of the C3H mouse have a higher average DNA content but similar platelet count, MPV, and megakaryocyte size and concentration as those of most other mouse strains. These results suggest that the number of platelets produced per unit of C3H megakaryocyte DNA is less than that for other mice.
到目前为止，在已检测的物种中，正常骨髓巨核细胞的DNA含量模式通常被报道为16N。在本报告中，我们描述了C3H小鼠中的一个例外情况，其巨核细胞的DNA含量模式为32N。雌性C3H/HEN小鼠的平均DNA含量分布为：8N占14%，16N占37%，32N占43%，64N占6%。雄性C3H/HEN小鼠中32N和64N巨核细胞的比例略高于雌性（平均DNA含量分布为：8N占12%，16N占29%，32N占47%，64N占12%）。所检测的其他11种小鼠品系均以16N作为巨核细胞DNA含量模式，尽管不同多倍体DNA类别的比例存在一些品系差异。在所有评估的12种品系中，巨核细胞大小相似，C3H/HEN小鼠的平均血小板体积（MPV）仅与所分析的其他4种品系中的1种不同。C3H/HEN小鼠的血小板计数与6种小鼠相似，略低于但显著低于所检测的其他5种小鼠品系。与所研究的其他小鼠品系的巨核细胞浓度相比，C3H/HEN小鼠的巨核细胞浓度与7种小鼠相似，比1种略高，比3种略低。C57BL/6和C3H/HEN小鼠正反交的后代巨核细胞DNA分布介于亲本品系之间，这表明某些成分的基因剂量较高是C3H小鼠巨核细胞DNA含量分布表型右移的原因。C3H/HEN小鼠以及用作比较品系的CBA/CAJ小鼠中，32N和64N巨核细胞的比例在急性血小板减少时增加。总之，C3H小鼠的巨核细胞平均DNA含量较高，但血小板计数、MPV、巨核细胞大小和浓度与大多数其他小鼠品系相似。这些结果表明，每单位C3H巨核细胞DNA产生的血小板数量少于其他小鼠。
