Chair for Proteomics and Bioanalytics, Center of Life and Food Sciences Weihenstephan, Technische Universität München, Freising, Germany.
Proteomics. 2013 Sep;13(17):2552-62. doi: 10.1002/pmic.201300076. Epub 2013 Jul 30.
The field of proteomics continues to be driven by improvements in analytical technology, notably in peptide separation, quantitative MS, and informatics. In this study, we have characterized a hybrid linear ion trap high field Orbitrap mass spectrometer (Orbitrap Elite) for proteomic applications. The very high resolution available on this instrument allows 95% of all peptide masses to be measured with sub-ppm accuracy that in turn improves protein identification by database searching. We further confirm again that mass accuracy in tandem mass spectra is a valuable parameter for improving the success of protein identification. The new CID rapid scan type of the Orbitrap Elite achieves similar performance as higher energy collision induced dissociation fragmentation and both allow the identification of hundreds of proteins from as little as 0.1 ng of protein digest on column. The new instrument outperforms its predecessor the Orbitrap Velos by a considerable margin on each metric assessed that makes it a valuable and versatile tool for MS-based proteomics.
蛋白质组学领域继续受到分析技术的改进推动，尤其是在肽分离、定量 MS 和信息学方面。在这项研究中，我们对一种混合线性离子阱高场轨道阱质谱仪（Orbitrap Elite）进行了蛋白质组学应用的特征描述。该仪器具有非常高的分辨率，可将 95%的所有肽质量以亚 ppm 的精度进行测量，从而通过数据库搜索提高蛋白质鉴定的成功率。我们进一步证实，串联质谱中的质量精度是提高蛋白质鉴定成功率的一个有价值的参数。Orbitrap Elite 的新型 CID 快速扫描类型与更高能量碰撞诱导解离碎裂具有相似的性能，两者都允许从柱上仅 0.1ng 的蛋白质消化物中鉴定数百种蛋白质。新仪器在每个评估指标上都明显优于其前代产品 Orbitrap Velos，使其成为基于 MS 的蛋白质组学的一种有价值且多功能的工具。
