Miyazawa T, Fujimoto K, Oikawa S
Department of Food Chemistry, Tohoku University, Sendai, Japan.
Biomed Chromatogr. 1990 May;4(3):131-4. doi: 10.1002/bmc.1130040312.
A high performance liquid chromatographic system with chemiluminescence detection (HPLC-CL) was used for determining phospholipid hydroperoxides in human plasma low density lipoprotein (LDL). This system involved separation of phospholipids from LDL-total lipids with normal phase silica gel HPLC and post-column detection of hydroperoxide-dependent chemiluminescence produced by luminol oxidation during the reaction of hydroperoxide with cytochrome c-haeme. By using HPLC-CL, we could detect phosphatidylcholine hydroperoxide (PCOOH) in human plasma LDL, and the LDL-PCOOH concentration was significantly higher in patients with atherosclerosis and hyperlipidemia than that of healthy volunteers. The LDL-PCOOH level was proportional to the plasma total cholesterol concentration.
采用带化学发光检测的高效液相色谱系统（HPLC-CL）测定人血浆低密度脂蛋白（LDL）中的磷脂氢过氧化物。该系统包括用正相硅胶HPLC从LDL总脂质中分离磷脂，以及柱后检测氢过氧化物与细胞色素c-血红素反应过程中鲁米诺氧化产生的依赖氢过氧化物的化学发光。通过HPLC-CL，我们能够检测人血浆LDL中的磷脂酰胆碱氢过氧化物（PCOOH），动脉粥样硬化和高脂血症患者的LDL-PCOOH浓度显著高于健康志愿者。LDL-PCOOH水平与血浆总胆固醇浓度成正比。
