Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Cold Spring Harb Perspect Biol. 2013 Sep 1;5(9):a010199. doi: 10.1101/cshperspect.a010199.
Poxviruses are large, enveloped viruses that replicate in the cytoplasm and encode proteins for DNA replication and gene expression. Hairpin ends link the two strands of the linear, double-stranded DNA genome. Viral proteins involved in DNA synthesis include a 117-kDa polymerase, a helicase-primase, a uracil DNA glycosylase, a processivity factor, a single-stranded DNA-binding protein, a protein kinase, and a DNA ligase. A viral FEN1 family protein participates in double-strand break repair. The DNA is replicated as long concatemers that are resolved by a viral Holliday junction endonuclease.
痘病毒是大型包膜病毒，在细胞质中复制，并编码用于 DNA 复制和基因表达的蛋白质。发夹末端连接线性双链 DNA 基因组的两条链。参与 DNA 合成的病毒蛋白包括 117kDa 聚合酶、解旋酶-引发酶、尿嘧啶 DNA 糖基化酶、持续因子、单链 DNA 结合蛋白、蛋白激酶和 DNA 连接酶。一种病毒 FEN1 家族蛋白参与双链断裂修复。DNA 作为长的串联体复制，由病毒 Holliday 连接内切酶解决。
