Nagy Gergely, Kovács László, Ünnep Renáta, Zsiros Ottó, Almásy László, Rosta László, Timmins Peter, Peters Judith, Posselt Dorthe, Garab Győző
Paul Scherrer Institute, Laboratory for Neutron Scattering, 5232 Villigen PSI, Switzerland.
Eur Phys J E Soft Matter. 2013 Jul;36(7):69. doi: 10.1140/epje/i2013-13069-0. Epub 2013 Jul 11.
We demonstrate the power of time-resolved small-angle neutron scattering experiments for the investigation of the structure and structural reorganizations of multilamellar photosynthetic membranes. In addition to briefly summarizing our results on thylakoid membranes isolated from higher plants and in unicellular organisms, we discuss the advantages and technical and methodological limitations of time-resolved SANS. We present a detailed and more systematical investigation of the kinetics of light-induced structural reorganizations in isolated spinach thylakoid membranes, which show how changes in the repeat distance and in the long-range order of the multilamellar membranes can be followed with a time resolution of seconds. We also present data from comparative measurements performed on thylakoid membranes isolated from tobacco.
我们展示了时间分辨小角中子散射实验在研究多层光合膜的结构和结构重组方面的强大功能。除了简要总结我们关于从高等植物和单细胞生物中分离出的类囊体膜的研究结果外，我们还讨论了时间分辨小角中子散射的优势以及技术和方法上的局限性。我们对分离的菠菜类囊体膜中光诱导结构重组的动力学进行了详细且更系统的研究，该研究展示了如何以秒级的时间分辨率追踪多层膜的重复距离和长程有序性的变化。我们还展示了对从烟草中分离出的类囊体膜进行的对比测量数据。
