Université de Bordeaux, ISVV, EA 3675 GESVAB, 33882, Villenave d'Ornon, France.
Phytochem Anal. 2013 Sep-Oct;24(5):446-52. doi: 10.1002/pca.2447. Epub 2013 Jul 9.
Grapes are one of the most important fruit crops in the world. The quality of red grape berries greatly depends on skin colour, mainly due to the anthocyanin profile. Today, the American Vitis species have the greatest potential for breeding work. They have multiple resistance properties in comparison with Vitis vinifera but little is known about their anthocyanin content.
To determine the anti-oxidant properties and anthocyanin profile of two American species, Vitis candicans and Vitis doaniana, by using LC-MS(n) and LC-NMR.
Grape extracts were prepared by extraction of berry skins with acidified methanol. The complete structure elucidation of the individual anthocyanins was performed with LC-MS(n) , LC-NMR and NMR experiments. Individual anthocyanins in the extracts were quantified by using malvidin glucoside as external standard. The anti-oxidant activities of grape skin extracts were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging and oxygen radical absorbance capacity (ORAC) assays.
By using LC-MS(n) and LC-NMR experiments, 30 anthocyanins were identified and quantified in the two Vitis species, including two new cis-p-coumaroyl derivatives. Vitis candicans and V. doaniana showed significant differences in their anthocyanin profile. These two Vitis species possess low-to-medium anti-oxidant activities in comparison with V. vinifera.
The profiles of 30 anthocyanins were established unambiguously in two American Vitis species.
葡萄是世界上最重要的水果作物之一。红葡萄浆果的品质在很大程度上取决于果皮颜色，主要是由于花色苷的分布。如今，美洲葡萄品种在培育工作中具有最大的潜力。与欧亚葡萄品种相比，它们具有多种抗性特性，但对其花色苷含量知之甚少。
采用 LC-MS(n) 和 LC-NMR 法测定两种美洲种葡萄，即 Vitis candicans 和 Vitis doaniana 的抗氧化特性和花色苷图谱。
采用酸化甲醇提取浆果果皮的方法制备葡萄提取物。通过 LC-MS(n)、LC-NMR 和 NMR 实验完成了对单个花色苷的完整结构阐明。采用矢车菊素葡萄糖苷作为外标，对提取物中的单个花色苷进行定量。通过 1,1-二苯基-2-苦基肼（DPPH•）自由基清除和氧自由基吸收能力（ORAC）测定法评估葡萄皮提取物的抗氧化活性。
通过 LC-MS(n) 和 LC-NMR 实验，在两种葡萄品种中鉴定和定量了 30 种花色苷，包括两种新的顺式-对香豆酰衍生物。Vitis candicans 和 V. doaniana 在花色苷图谱上存在显著差异。与 V. vinifera 相比，这两个葡萄品种的抗氧化活性较低。
明确建立了两种美洲种葡萄中的 30 种花色苷图谱。
