Université de Bordeaux, ISVV, EA 3675 GESVAB, 33882 Villenave d'Ornon, France.
Anal Chim Acta. 2012 Jun 30;732:145-52. doi: 10.1016/j.aca.2011.11.060. Epub 2011 Dec 7.
The composition and concentration of anthocyanins of grape berry skins were analyzed in order to assess phenotypic variation between four grape wine varieties belonging to 4 different species: Vitis vinifera, Vitis amurensis, Vitis cinerea and Vitis X champinii. High-performance liquid chromatography coupled to mass spectrometry (LC-MS) and NMR spectroscopy (LC-NMR) were used to separate and identify the structure of anthocyanins present in these species. Combination of LC-MS and LC-NMR data resulted in the identification of 33 anthocyanins. In particular, newly reported cis isomers of p-coumaric-derivatives were identified (petunidin-, peonidin- and malvidin-3-(6-p-coumaroyl)-5-diglucoside). In V. cinerea and V. vinifera, anthocyanins were monoglucoside derivatives whereas in V. amurensis and V. X champinii, both mono- and diglucoside derivatives were identified. Malvidin-, delphinidin- and petunidin-derivatives were, respectively, the most abundant components in V. cinerea and V. vinifera, V. amurensis and V. X champinii.
为了评估属于 4 个不同物种的 4 种葡萄酒品种（葡萄、山葡萄、蛇龙珠和山葡萄）之间的表型变异，分析了葡萄果皮中花色苷的组成和浓度。采用高效液相色谱-质谱联用（LC-MS）和核磁共振波谱（LC-NMR）分离和鉴定这些物种中花色苷的结构。LC-MS 和 LC-NMR 数据的组合鉴定出 33 种花色苷。特别是，鉴定出了新报道的对香豆酸衍生的顺式异构体（矢车菊素、芍药素和锦葵素-3-（6-对香豆酰基）-5-双葡萄糖苷）。在蛇龙珠和葡萄中，花色苷为单葡萄糖苷衍生物，而在山葡萄和山葡萄中，鉴定出了单葡萄糖苷和双葡萄糖苷衍生物。矢车菊素、飞燕草素和锦葵素衍生物分别是蛇龙珠和葡萄、山葡萄和山葡萄中含量最丰富的成分。
