Miyazaki T, Matsuzaki K
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Endocrinol Jpn. 1990 Feb;37(1):103-12. doi: 10.1507/endocrj1954.37.103.
After the subcutaneous injection of a large amount of aldosterone into a male rabbit, urine was collected for 24 h. The preliminary separation of urinary aldosterone metabolites was carried out by means of DEAE-Sephadex A-25 column chromatography. Each fraction obtained was further purified by reversed phase high pressure liquid chromatography. Purified materials were then analyzed by fast atom bombardment mass spectrometry and infrared spectroscopy. Thus, tetrahydroaldosterone glucuronide and tetrahydroaldosterone sulfate were detected as urinary aldosterone metabolites. These results confirmed our previously published data, where the nature of conjugating groups was determined indirectly. Furthermore, hydroxyaldosterone was identified as a urinary aldosterone metabolite.
给一只雄性兔子皮下注射大量醛固酮后，收集其24小时尿液。采用二乙氨基乙基葡聚糖A - 25柱色谱法对尿醛固酮代谢物进行初步分离。所得到的每个馏分再通过反相高压液相色谱法进一步纯化。然后用快原子轰击质谱法和红外光谱法对纯化后的物质进行分析。结果检测到四氢醛固酮葡糖醛酸苷和四氢醛固酮硫酸盐为尿醛固酮代谢物。这些结果证实了我们之前发表的数据，在那些数据中结合基团的性质是间接确定的。此外，羟基醛固酮被鉴定为一种尿醛固酮代谢物。
