Morris D J, Latif S A, Conca T J, Wei L T, Watlington C O, Kirk D N, Toms H C, Shackleton C H
Department of Laboratory Medicine, Miriam Hospital, Providence, RI 02906.
Steroids. 1990 Nov;55(11):482-7. doi: 10.1016/0039-128x(90)90084-o.
Incubation of aldosterone with confluent layers of A6 (toad kidney) cells leads to its hydroxylation at the 6 beta-position. 6 beta-Hydroxyaldosterone is the major metabolite when the incubation is carried out at pH 6.8, whereas the product comprises 6 beta-hydroxy-17-isoaldosterone accompanied by some 6 beta-hydroxyapoaldosterone at pH 7.4. All products were identified by high-field 1H nuclear magnetic resonance spectroscopy. Control experiments indicated that the side-chain isomerization to form the 17-iso and apo derivatives occurs after the cytochrome P 450-dependent synthesis of 6 beta-hydroxyaldosterone.
将醛固酮与汇合的A6（蟾蜍肾）细胞层一起孵育会导致其在6β位发生羟基化。当在pH 6.8下进行孵育时，6β-羟基醛固酮是主要代谢产物，而在pH 7.4时，产物包括6β-羟基-17-异醛固酮以及一些6β-羟基阿朴醛固酮。所有产物均通过高场1H核磁共振光谱法鉴定。对照实验表明，形成17-异和阿朴衍生物的侧链异构化发生在细胞色素P 450依赖性合成6β-羟基醛固酮之后。
