Murakami M, Kudo I, Nakamura H, Yokoyama Y, Mori H, Inoue K
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
FEBS Lett. 1990 Jul 30;268(1):113-6. doi: 10.1016/0014-5793(90)80986-s.
Appreciable phospholipase A2 activity was detected in a hind paw homogenate from rats with adjuvant arthritis or carrageenan-induced edema. The activity was neutralized by treatment with antibody raised against rat platelet secretory phospholipase A2, indicating that 14-kDa group II phospholipase A2 was induced in the hind paw during the process of inflammation. Injection of purified rat platelet phospholipase A2 into the hind paw of rats with adjuvant-induced arthritis resulted in exacerbation of edema in a dose-dependent manner, whereas no effect was observed on either normal rats or animals with carrageenan-induced edema under the same experimental conditions. These results suggest the importance of mammalian group II phospholipase A2 in the pathogenesis of some types of inflammation.
在佐剂性关节炎或角叉菜胶诱导水肿的大鼠后爪匀浆中检测到明显的磷脂酶A2活性。用抗大鼠血小板分泌型磷脂酶A2产生的抗体处理可中和该活性，表明在炎症过程中后爪诱导产生了14-kDa的II型磷脂酶A2。将纯化的大鼠血小板磷脂酶A2注射到佐剂性关节炎大鼠的后爪中，会导致水肿以剂量依赖性方式加剧，而在相同实验条件下，对正常大鼠或角叉菜胶诱导水肿的动物均未观察到影响。这些结果表明哺乳动物II型磷脂酶A2在某些类型炎症的发病机制中具有重要作用。
