Högfeldt Therese, Johnsson Per, Grandér Dan, Bahnassy Abeer A, Porwit Anna, Eid Salem, Österborg Anders, Zekri Abdel-Rahman N, Lundahl Joachim, Khaled Mustafa Hussein, Mellstedt Håkan, Moshfegh Ali
Department of Oncology and Pathology, Karolinska Institutet , Stockholm , Sweden.
Leuk Lymphoma. 2014 May;55(5):1158-65. doi: 10.3109/10428194.2013.824077. Epub 2013 Aug 31.
Abstract Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous disease, and infectious agents are suspected to be involved in the tumorigenesis of DLBCL. MicroRNAs (miRNAs) are non-coding RNAs modulating protein expression. We compared miRNA expression profiles in lymph node tissues of patients with DLBCL of the activated B-cell like (ABC) type from two geographical areas with different background exposures, Sweden and Egypt. We showed previously that DLBCL tissues of the ABC-type in Swedish patients had a higher expression of signal transducer and activator of transcription 3 (STAT3) compared to Egyptian patients. Here, we analyzed the involvement of miRNAs in STAT3 regulation. miR-1234 was significantly up-regulated in Egyptian patients with DLBCL compared to Swedish patients (p < 0.03). The miR-1234 expression level correlated inversely with the expression of STAT3. The Stat3 protein was down-regulated in cells transfected with miR-1234, suggesting that STAT3 might be a potential target for miR-1234. miR-1234 and STAT3 might be involved in the tumorigenesis of DLBCL of ABC type and possibly associated with environmental background exposure.
摘要 弥漫性大B细胞淋巴瘤（DLBCL）是一种异质性疾病，怀疑感染因子参与了DLBCL的肿瘤发生过程。微小RNA（miRNA）是调节蛋白质表达的非编码RNA。我们比较了来自瑞典和埃及这两个具有不同背景暴露的地理区域的活化B细胞样（ABC）型DLBCL患者淋巴结组织中的miRNA表达谱。我们之前表明，与埃及患者相比，瑞典患者ABC型的DLBCL组织中信号转导和转录激活因子3（STAT3）的表达更高。在此，我们分析了miRNA在STAT3调控中的作用。与瑞典患者相比，埃及DLBCL患者中miR-1234显著上调（p < 0.03）。miR-1234的表达水平与STAT3的表达呈负相关。在用miR-1234转染的细胞中Stat3蛋白被下调，这表明STAT3可能是miR-1234的潜在靶点。miR-1234和STAT3可能参与ABC型DLBCL的肿瘤发生，并且可能与环境背景暴露有关。
