Zhang Peng, Liu Naicheng, Xue Mingyang, Zhang Mengjie, Liu Wei, Xu Chen, Fan Yuding, Meng Yan, Zhang Qinghua, Zhou Yong
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Antioxidants (Basel). 2023 Feb 6;12(2):391. doi: 10.3390/antiox12020391.
β-Sitosterol, which is used extensively in pharmaceuticals, nutraceuticals, and cosmetics, has high nutritional value along with immunomodulatory and anti-inflammatory properties. In this study, we investigated the antioxidant and anti-inflammatory effects of β-sitosterol in zebrafish and explored the associated molecular mechanisms. In an in vivo antioxidant experiment, zebrafish () larvae were treated with different concentrations of β-sitosterol and then exposed to a nonlethal concentration of CuSO to induce oxidative stress. Treatment with β-sitosterol at 70 or 100 μg/mL significantly reduced CuSO-induced oxidative stress in the zebrafish, demonstrating the strong antioxidant activity of β-sitosterol. Treatment with β-sitosterol protected zebrafish larvae against oxidative damage from CuSO by upregulating the expressions of and . In a zebrafish model of inflammation, pretreatment with β-sitosterol before CuSO4 exposure inhibited neutrophil recruitment and damage to lateral line neuromasts, indicating a potent anti-inflammatory effect derived from reductions in the expressions of and . The results demonstrate the antioxidative and anti-inflammatory activities of β-sitosterol and suggest that β-sitosterol may be useful for the treatment of various inflammatory diseases.
β-谷甾醇广泛应用于制药、营养保健品和化妆品领域，具有很高的营养价值以及免疫调节和抗炎特性。在本研究中，我们研究了β-谷甾醇对斑马鱼的抗氧化和抗炎作用，并探索了相关的分子机制。在一项体内抗氧化实验中，用不同浓度的β-谷甾醇处理斑马鱼（）幼体，然后使其暴露于非致死浓度的硫酸铜中以诱导氧化应激。用70或100μg/mL的β-谷甾醇处理可显著降低硫酸铜诱导的斑马鱼氧化应激，证明了β-谷甾醇具有强大的抗氧化活性。用β-谷甾醇处理通过上调和的表达保护斑马鱼幼体免受硫酸铜的氧化损伤。在炎症斑马鱼模型中，在暴露于硫酸铜之前用β-谷甾醇预处理可抑制中性粒细胞募集和侧线神经丘损伤，表明其抗炎作用源于和表达的降低。结果证明了β-谷甾醇的抗氧化和抗炎活性，并表明β-谷甾醇可能对治疗各种炎症性疾病有用。
