Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
Int J Mol Sci. 2023 May 10;24(10):8518. doi: 10.3390/ijms24108518.
Long-term or excessive oxidative stress can cause serious damage to fish. Squalene can be added to feed as an antioxidant to improve the body constitution of fish. In this study, the antioxidant activity was detected by 2,2-diphenyl-1-acrylhydrazyl (DPPH) test and fluorescent probe (dichloro-dihydro-fluorescein diacetate). Transgenic (: ) zebrafish were used to evaluate the effect of squalene on CuSO-induced inflammatory response. Quantitative real-time reverse transcription polymerase chain reaction was used to examine the expression of immune-related genes. The DPPH assay demonstrated that the highest free radical scavenging exerted by squalene was 32%. The fluorescence intensity of reactive oxygen species (ROS) decreased significantly after 0.7% or 1% squalene treatment, and squalene could exert an antioxidative effect in vivo. The number of migratory neutrophils in vivo was significantly reduced after treatment with different doses of squalene. Moreover, compared with CuSO treatment alone, treatment with 1% squalene upregulated the expression of by 2.5-foldand by 1.3-fold to protect zebrafish larvae against CuSO-induced oxidative damage. Moreover, treatment with 1% squalene significantly downregulated the expression of and . This study showed that squalene has potential as an aquafeed additive to provide both anti-inflammatory and antioxidative properties.
长期或过度的氧化应激会对鱼类造成严重损害。角鲨烯可以作为一种抗氧化剂添加到饲料中，以改善鱼类的体质。在这项研究中，通过 2,2-二苯基-1-丙烯酰肼（DPPH）试验和荧光探针（二氯二氢荧光素二乙酸酯）检测了角鲨烯的抗氧化活性。使用转基因（：）斑马鱼来评估角鲨烯对 CuSO 诱导的炎症反应的影响。采用定量实时反转录聚合酶链反应检测免疫相关基因的表达。DPPH 试验表明，角鲨烯的最高自由基清除率为 32%。经过 0.7%或 1%角鲨烯处理后，活性氧（ROS）的荧光强度显著降低，角鲨烯在体内具有抗氧化作用。体内迁移的中性粒细胞数量在不同剂量的角鲨烯处理后显著减少。此外，与单独使用 CuSO 处理相比，用 1%角鲨烯处理可使 基因的表达上调 2.5 倍， 基因的表达上调 1.3 倍，从而保护斑马鱼幼虫免受 CuSO 诱导的氧化损伤。此外，用 1%角鲨烯处理可显著下调 基因和 的表达。这项研究表明，角鲨烯具有作为水产饲料添加剂的潜力，可提供抗炎和抗氧化特性。
