State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, People's Republic of China.
J Agric Food Chem. 2013 Jul 24;61(29):7165-71. doi: 10.1021/jf401748a. Epub 2013 Jul 10.
Using solvent sublation (SS), a novel pretreatment method for separating and concentrating antioxidants and ultraviolet absorbers from plastic beverage packaging was developed, and these target compounds were quantitatively analyzed by high-performance liquid chromatography (HPLC). In the pretreatment section, the effects of the sublation solvent, solution pH, NaCl concentration, nitrogen flow rate, sublation time, and light condition on the sublation efficiency were investigated in detail and the optimal conditions of the solvent sublation process were selected. The analytical method of SS-HPLC showed good linearity in the range from 0.33 to 667 ng/mL with good presenting regression coefficients (0.9995 ≥ R(2) ≥ 0.9972). Low limits of detection (LODs) of 0.34-1.25 ng/mL and limits of quantification (LOQs) of 1.13-4.15 ng/mL were achieved. The mean recoveries were in the range from 88.73 to 107.65% at 20, 30, and 40 ng/mL spiked levels, and the relative standard deviations (RSDs) were in the range from 2.16 to 10.55%.
采用溶剂浮选（SS）法，开发了一种从塑料饮料包装中分离和浓缩抗氧化剂和紫外线吸收剂的新型预处理方法，并通过高效液相色谱（HPLC）对这些目标化合物进行定量分析。在预处理部分，详细考察了浮选溶剂、溶液 pH 值、NaCl 浓度、氮气流速、浮选时间和光照条件对浮选效率的影响，选择了溶剂浮选过程的最佳条件。SS-HPLC 的分析方法在 0.33-667ng/mL 范围内具有良好的线性关系，呈现出良好的回归系数（0.9995≥R(2)≥0.9972）。检测限（LOD）低至 0.34-1.25ng/mL，定量限（LOQ）低至 1.13-4.15ng/mL。在 20、30 和 40ng/mL 加标水平下，平均回收率在 88.73%-107.65%之间，相对标准偏差（RSD）在 2.16%-10.55%之间。
