Çabuk Hasan, Köktürk Mustafa
Department of Chemistry, Faculty of Arts and Sciences, Bülent Ecevit University, 67100 Zonguldak, Turkey.
ScientificWorldJournal. 2013 Jun 17;2013:414398. doi: 10.1155/2013/414398. Print 2013.
A simple and efficient method was established for the determination of synthetic antioxidants in beverages by using dispersive liquid-liquid microextraction combined with high-performance liquid chromatography with ultraviolet detection. Butylated hydroxy toluene, butylated hydroxy anisole, and tert-butylhydroquinone were the antioxidants evaluated. Experimental parameters including extraction solvent, dispersive solvent, pH of sample solution, salt concentration, and extraction time were optimized. Under optimal conditions, the extraction recoveries ranged from 53 to 96%. Good linearity was observed by the square of correlation coefficients ranging from 0.9975 to 0.9997. The relative standard deviations ranged from 1.0 to 5.2% for all of the analytes. Limits of detection ranged from 0.85 to 2.73 ng mL(-1). The method was successfully applied for determination of synthetic antioxidants in undiluted beverage samples with satisfactory recoveries.
建立了一种简单高效的方法，采用分散液液微萃取结合带紫外检测的高效液相色谱法测定饮料中的合成抗氧化剂。所评估的抗氧化剂为丁基羟基甲苯、丁基羟基茴香醚和叔丁基对苯二酚。对萃取溶剂、分散溶剂、样品溶液的pH值、盐浓度和萃取时间等实验参数进行了优化。在最佳条件下，萃取回收率在53%至96%之间。相关系数平方在0.9975至0.9997之间呈现良好的线性关系。所有分析物的相对标准偏差在1.0%至5.2%之间。检测限在0.85至2.73 ng mL(-1)之间。该方法成功应用于未稀释饮料样品中合成抗氧化剂的测定，回收率令人满意。
