Xie S, Broermann D M, Nephew K P, Bishop M D, Pope W F
Ohio State University, Columbus 43210.
J Anim Sci. 1990 Jul;68(7):2027-33. doi: 10.2527/1990.6872027x.
Two experiments were conducted to examine how oocyte maturation and fertilization influence zygotic diversity in swine. In the first experiment, the distribution of oocyte maturation was compared to that of zygotic development. Oocytes were aspirated from follicles of 31 gilts and classified into stages of meiosis. Zygotes were flushed from oviducts of 19 additional gilts and classified into stages of meiosis and fertilization. The second experiment examined whether the time from ovulation to fertilization was constant among all oocytes. To test this premise, four to six oocytes from follicles of 10 mated gilts were aspirated just before or during ovulation, stained and transferred back into the oviducts of these same gilts. Zygotes were recovered 10 h later to determine whether the first oocytes ovulated were the more developed zygotes and, conversely, whether the last oocytes to be ovulated represented the lesser developed contemporaries. The skewed (P less than .05) distribution of oocyte maturation was similar to that of zygotic development. Regression of the frequency distribution describing early oocyte maturation resulted in a line with a slope (.59) that was similar to the slope (.58) of the regressed distribution of zygotic development. Likewise, the order of ovulation and order of subsequent stages of zygotic development were similar. These data suggest that variation in zygotic development in swine was due to variability in oogenesis; the time from ovulation to fertilization appeared to be constant.
进行了两项实验，以研究卵母细胞成熟和受精如何影响猪的合子多样性。在第一个实验中，将卵母细胞成熟的分布与合子发育的分布进行了比较。从31头后备母猪的卵泡中吸出卵母细胞，并将其分类为减数分裂阶段。从另外19头后备母猪的输卵管中冲洗出合子，并将其分类为减数分裂和受精阶段。第二个实验研究了所有卵母细胞从排卵到受精的时间是否恒定。为了验证这一前提，在排卵前或排卵期间从10头交配后备母猪的卵泡中吸出4至6个卵母细胞，进行染色，然后再放回这些后备母猪的输卵管中。10小时后回收合子，以确定首先排卵的卵母细胞是否是发育更成熟的合子，反之，最后排卵的卵母细胞是否代表发育较不成熟的同期卵母细胞。卵母细胞成熟的偏态分布（P小于0.05）与合子发育的分布相似。描述早期卵母细胞成熟的频率分布回归得到的直线斜率为0.59，与合子发育回归分布的斜率0.58相似。同样，排卵顺序与合子发育后续阶段的顺序相似。这些数据表明，猪合子发育的差异是由于卵子发生的变异性；从排卵到受精的时间似乎是恒定的。
