Food and Feed Safety Unit, Southern Plains Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, College Station, Texas 77845, USA.
Int Microbiol. 2012 Dec;15(4):185-9. doi: 10.2436/20.1501.01.171.
The aim of this work was to determine the destructive activity of dextranase, lactoferrin, and lysozyme, against single species biofilms composed of either Klebsiella pneumoniae subsp. pneumoniae or Escherichia coli using the MBEC Assay. Luminescence measurements based on quantitation of the ATP present were used to determine the amount of biofilm elimination and correlated with quantity of live bacteria present in the sample. The data were analyzed employing a two-way ANOVA and Bonferroni post-test. Treatments resulted in percentage reductions of E. coli biofilms ranging from 73 to 98%. Lactoferrin (40 microg/ml) produced a significantly higher-percentage reduction than lysozyme (10 microg/ml) (P < 0.05), no other significant differences occurred. Similar treatments resulted in percentage reductions of K. pneumoniae subsp. pneumoniae biofilms ranging from 51 to 100%. Dextranase treatments produced a significantly lower percentage reduction than all other materials (P < 0.05), no other significant differences occurred. No material was capable of complete destruction of both single species biofilms; however, low concentrations of lactoferrin and lysozyme each removed 100% of the K. pneumoniae subsp. pneumoniae biofilm. Low concentrations of lactoferrin or lysozyme might be beneficial to prevent biofilm formation by K. pneumoniae subsp. pneumoniae.
这项工作的目的是使用 MBEC 测定法来确定葡聚糖酶、乳铁蛋白和溶菌酶对由肺炎克雷伯菌亚种或大肠杆菌组成的单一物种生物膜的破坏活性。基于存在的 ATP 的定量的发光测量用于确定生物膜消除的量，并与样品中存在的活细菌的量相关联。使用双向 ANOVA 和 Bonferroni 后测试分析数据。处理导致大肠杆菌生物膜的百分比减少范围为 73%至 98%。乳铁蛋白（40μg/ml）产生的百分比降低显著高于溶菌酶（10μg/ml）（P<0.05），没有发生其他显著差异。类似的处理导致肺炎克雷伯菌亚种生物膜的百分比减少范围为 51%至 100%。葡聚糖酶处理产生的百分比降低显著低于所有其他材料（P<0.05），没有发生其他显著差异。没有一种材料能够完全破坏两种单一物种生物膜；然而，低浓度的乳铁蛋白和溶菌酶各自去除了 100%的肺炎克雷伯菌亚种生物膜。低浓度的乳铁蛋白或溶菌酶可能有益于预防肺炎克雷伯菌亚种的生物膜形成。
