Bukharin O V, Sgibnev A V
Zh Mikrobiol Epidemiol Immunobiol. 2012 May-Jun(3):70-3.
Study of influence of low concentrations of active forms of oxygen on the ability of bacteria to form biofilms and their adhesive characteristics.
Modification of the studied properties of bacteria was performed by treatment of the bacteria with hydrogen peroxide or hydroxyl radicals at concentrations of 0.5 mM that causes death of 5 - 7% of cells and non-lethal - 0.05 and 0.005 mM. Adhesive characteristics of Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Klebsiella pneumoniae bacteria were determined by using human erythrocytes. The degree of biofilm formation in plates modified by collagen from human fibroblasts was determined by the amount of bound crystal violet.
A significant decrease (7 - 17%) of the adhesion of E. coli, S. aureus, K. pneumoniae to erythrocytes under the influence of hydrogen peroxide and hydroxyl radicals at the concentrations of 0.5 and 0.05 mM when compared with the control was detected. 11 - 40% stimulation of E. coli, S. aureus, K. pneumoniae biofilm formation by active forms of oxygen in all the used concentrations with simultaneous insignificant biomass growth increase was noted. Active forms of oxygen did not influence significantly the studied properties of B. subtilis.
Active oxygen forms have regulatory influence on the character of interaction of bacteria with surfaces colonized by them, that determines structural-functional state of microbial biocenoses.
研究低浓度活性氧对细菌形成生物膜能力及其黏附特性的影响。
通过用过氧化氢或羟基自由基处理细菌来改变所研究的细菌特性，处理浓度分别为0.5 mM（导致5 - 7%的细胞死亡）、非致死浓度0.05 mM和0.005 mM。使用人红细胞测定大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌和肺炎克雷伯菌的黏附特性。通过测定结合结晶紫的量来确定人成纤维细胞胶原修饰平板中生物膜的形成程度。
与对照组相比，在0.5 mM和0.05 mM浓度的过氧化氢和羟基自由基作用下，大肠杆菌、金黄色葡萄球菌、肺炎克雷伯菌对红细胞的黏附显著降低（7 - 17%）。在所有使用浓度下，活性氧对大肠杆菌、金黄色葡萄球菌、肺炎克雷伯菌生物膜形成有11 - 40%的刺激作用，同时生物量增长不显著。活性氧对枯草芽孢杆菌的所研究特性无显著影响。
活性氧形式对细菌与它们所定殖表面的相互作用特性具有调节作用，这决定了微生物生物群落的结构 - 功能状态。
