使用[11C]氨基环己烷羧酸盐测量人脑中氨基酸的摄取和分布容积。

Use of [11C]aminocyclohexanecarboxylate for the measurement of amino acid uptake and distribution volume in human brain.

作者信息

Koeppe R A, Mangner T, Betz A L, Shulkin B L, Allen R, Kollros P, Kuhl D E, Agranoff B W

机构信息

Department of Internal Medicine, University of Michigan Medical School, Ann Arbor 48109-0552.

出版信息

J Cereb Blood Flow Metab. 1990 Sep;10(5):727-39. doi: 10.1038/jcbfm.1990.126.

DOI:10.1038/jcbfm.1990.126

PMID:2384544

Abstract

A quantitative positron emission tomographic (PET) method to measure amino acid blood-brain barrier (BBB) transport rate and tissue distribution volume (DV) has been developed using 11C-labeled aminocyclohexanecarboxylate (ACHC), a nonmetabolized amino acid analogue. Dynamic PET data were acquired as a series of 15 scans covering a total of 60 min and analyzed by means of a two-compartment, two-parameter model. Functional images were calculated for the amino acid transport rate constants across the BBB and the amino acid DV in the brain. Results show [11C]ACHC to have an influx rate constant in gray matter of approximately 0.03-0.04 ml g-1 min-1, indicating a single-pass extraction fraction of approximately 5-7%. The intersubject coefficient of variation was approximately 15% while intrasubject variability of repeat scans was only slightly greater than 5%. Studies were performed in 15 young normal volunteer control subjects, 5 elderly controls, 7 patients with probable Alzheimer's disease, and one patient with phenylketonuria. Results indicate that [11C]-ACHC will serve as the basis of a method for measuring amino acid transport rate and DV in the normal and pathological human brain.

摘要

一种使用¹¹C标记的氨基环己烷羧酸（ACHC，一种非代谢性氨基酸类似物）来测量氨基酸血脑屏障（BBB）转运速率和组织分布容积（DV）的定量正电子发射断层扫描（PET）方法已被开发出来。动态PET数据作为一系列共15次扫描获取，涵盖总共60分钟，并通过双室双参数模型进行分析。计算出了跨BBB的氨基酸转运速率常数和大脑中氨基酸DV的功能图像。结果显示，[¹¹C]ACHC在灰质中的流入速率常数约为0.03 - 0.04 ml g⁻¹ min⁻¹，表明单次通过提取率约为5 - 7%。受试者间变异系数约为15%，而重复扫描的受试者内变异性仅略大于5%。对15名年轻正常志愿者对照受试者、5名老年对照者、7名可能患有阿尔茨海默病的患者以及1名苯丙酮尿症患者进行了研究。结果表明，[¹¹C] - ACHC将作为一种测量正常和病理性人脑氨基酸转运速率和DV的方法的基础。